Motility of Pseudomonas aeruginosa contributes to SOS-inducible biofilm formation
Motility of Pseudomonas aeruginosa contributes to SOS-inducible biofilm formation
复制标题
铜绿假单胞菌的运动能力有助于 SOS 诱导的生物膜形成
DOI：
10.1016/j.resmic.2013.10.001
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发表时间：
2013-12-01
影响因子：
2.6
通讯作者：
Tao Weitao
中科院分区：
文献类型：
--
作者：
Chellappa, Shakinah T.;Maredia, Reshma;Tao Weitao
DNA损伤抗生素如环丙沙星通过自切割铜绿假单胞菌中的SOS-阻遏物莱克萨诱导生物膜形成和SOS反应。然而，生物膜SOS连接仍然知之甚少。用96孔和脂质生物膜测定法进行了研究。研究了环丙沙星对SOS突变体和野生型菌株的生物膜刺激的影响。在其中SOS被诱导的野生型中观察到的刺激在其中使莱克萨不可切割（LexAN）并且因此SOS不可诱导的突变体中减少。因此，刺激似乎涉及SOS。通过对生物膜外膜组分的亚蛋白质组学分析，探讨了诱导生物膜形成的可能机制。这些数据预测了莱克萨在鞭毛功能中的抑制作用。这一前提进行了测试，首先通过功能和形态学分析的鞭毛为基础的运动。环丙沙星处理的LexAN菌株鞭毛游泳运动能力下降。其次，进行运动性生物膜测定，其测试细胞迁移和生物膜形成。结果表明，野生型生物膜比LexAN显著增加。这些结果表明，莱克萨抑制的运动，这是在生物膜的发展的初始事件，有助于抑制SOS诱导的生物膜的形成。(C)2013年巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
DNA-damaging antibiotics such as ciprofloxacin induce biofilm formation and the SOS response through autocleavage of SOS-repressor LexA in Pseudomonas aeruginosa. However, the biofilm-SOS connection remains poorly understood. It was investigated with 96-well and lipid biofilm assays. The effects of ciprofloxacin were examined on biofilm stimulation of the SOS mutant and wild-type strains. The stimulation observed in the wild-type in which SOS was induced was reduced in the mutant in which LexA was made non-cleavable (LexAN) and thus SOS non-inducible. Therefore, the stimulation appeared to involve SOS. The possible mechanisms of inducible biofilm formation were explored by subproteomic analysis of outer membrane fractions extracted from biofilms. The data predicted an inhibitory role of LexA in flagellum function. This premise was tested first by functional and morphological analyses of flagellum-based motility. The flagellum swimming motility decreased in the LexAN strain treated with ciprofloxacin. Second, the motility-biofilm assay was performed, which tested cell migration and biofilm formation. The results showed that wild-type biofilm increased significantly over the LexAN. These results suggest that LexA repression of motility, which is the initial event in biofilm development, contributes to repression of SOS-inducible biofilm formation. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.