Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs.
Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs.
复制标题
新鉴定的 RNA 解旋酶 II/Gu 旁系同源物的基因组结构：假基因和多个选择性剪接 mRNA 的检测。
DOI：
10.1016/s0378-1119(01)00888-5
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发表时间：
2002
期刊：
影响因子：
3.5
通讯作者：
Sequitin,AnneMarie
中科院分区：
文献类型：
--
作者：
Valdez,BenignoC;Yang,Hushan;Hong,Eugene;Sequitin,AnneMarie
RNA解旋酶II/Gu（RH-II/Gu或DDX 21）是一种定位于核仁的DEAD盒酶，可能参与核糖体RNA的合成或加工。它有两个旁系同源物，RH-II/Guα和RH-II/Guβ，这两个基因都位于10号染色体上。它们相似的基因组结构表明这两个基因是由基因复制产生的。与匹配的肿瘤组织相比，这两种基因在一些正常人体组织中以更高的水平表达。RH-II/Guβ的假基因存在于染色体2、3和4上。未发现RH-II/Guα的假基因。外显子插入和外显子跳跃均对RH-II/Guβ基因表达具有转录后调节作用。未发现RH-II/Guα的选择性剪接。总体而言，结果表明，RH-II/Gu的两个旁系同源物由基因重复产生，但所产生的基因受到差异调节。
RNA helicase II/Gu (RH-II/Gu or DDX21) is a DEAD-box enzyme that localizes to the nucleoli and may be involved in ribosomal RNA synthesis or processing. It has two paralogues, RH-II/Guα and RH-II/Guβ, both genes of which are on chromosome 10. Their similar genomic structures suggest the two genes arose by gene duplication. Both genes are expressed at higher levels in some normal human tissues compared to matching tumor tissues. Pseudogenes for RH-II/Guβ exist on chromosomes 2, 3 and 4. No pseudogene was identified for RH-II/Guα. Both exon inclusion and exon skipping were found to post-transcriptionally regulate RH-II/Guβ gene expression. No alternative splicing was identified for RH-II/Guα. Overall, the results suggest that the two paralogues of RH-II/Gu arose by gene duplication but the resulting genes are differentially regulated.