PACAP modulates the consolidation and extinction of the contextual fear conditioning through NMDA receptors
PACAP modulates the consolidation and extinction of the contextual fear conditioning through NMDA receptors
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DOI：
10.1016/j.nlm.2014.11.014
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发表时间：
2015-02-01
影响因子：
2.7
通讯作者：
Izquierdo, I.
中科院分区：
文献类型：
--
作者：
Schmidt, S. D.;Myskiw, J. C.;Izquierdo, I.
腺苷酸环化酶激活多肽（PACAP）具有神经递质、神经营养和神经保护等多种生物学功能。此外，已经表明PACAP在调节学习和记忆以及谷氨酸信号传导中起作用。因此，在目前的研究中，我们研究了在海马CA 1区和基底外侧杏仁核（BLA）的作用，PACAP的巩固和灭绝的上下文恐惧条件反射（CFC）和PACAP和NMDA受体之间的相互作用。在训练或消退训练后，将插管植入海马CA 1区或BLA的雄性大鼠立即接受溶媒、PACAP-38（40 μ g/侧）、PACAP 6-38（40 μ g/侧）或PACAP 6-38加D-丝氨酸（50 μ g/侧）的CFC输注。24小时后，对动物进行3分钟保留试验。结果表明，在海马CA 1区，PACAP参与CFC的巩固和消退，而在BLA，PACAP只参与CFC的巩固。此外，这些结果表明，PACAP对CFC的巩固和消退的作用是通过谷氨酸NMDA受体介导的。(C)2014年爱思唯尔公司All rights reserved.
Pituitary adenylate cyclase-activating polypeptide (PACAP) has a broad spectrum of biological functions including neurotransmitter, neurotrophic and neuroprotective. Moreover, it has been suggested that PACAP plays a role in the modulation of learning and memory as well as on the modulation of glutamate signaling. Thus, in the current study we investigated in the CA1 region of hippocampus and in the basolateral amygdala (BLA) the role of PACAP in the consolidation and extinction of contextual fear conditioning (CFC) and the interaction between PACAP and NMDA receptors. Male rats with cannulae implanted in the CA1 region of the hippocampus or in the BLA received immediately after the training or extinction training of the CFC infusions of the Vehicle, PACAP-38 (40 pg/side), PACAP 6-38 (40 pg/side) or PACAP 6-38 plus D-serine (50 mu g/side). After 24 h, the animals were subjected to a 3-min retention test. The results indicated that in the CA1 region of hippocampus, PACAP participates in the consolidation and extinction of the CFC, and in the BLA, PACAP participates only in the consolidation of the CFC. Additionally, the results suggest that the action of PACAP on the consolidation and extinction of the CFC is mediated by the glutamate NMDA receptors. (C) 2014 Elsevier Inc. All rights reserved.