RECOMBINATION-DEFICIENT MUTANTS OF SALMONELLA-TYPHIMURIUM ARE AVIRULENT AND SENSITIVE TO THE OXIDATIVE BURST OF MACROPHAGES
RECOMBINATION-DEFICIENT MUTANTS OF SALMONELLA-TYPHIMURIUM ARE AVIRULENT AND SENSITIVE TO THE OXIDATIVE BURST OF MACROPHAGES
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DOI：
10.1111/j.1365-2958.1993.tb01184.x
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发表时间：
1993-03-01
影响因子：
3.6
通讯作者：
HEFFRON, F
中科院分区：
文献类型：
--
作者：
BUCHMEIER, NA;LIPPS, CJ;HEFFRON, F
在强毒鼠伤寒沙门氏菌14028s菌株中构建了recA和recBC基因的突变。recA和recBC突变体在小鼠体内均减毒。这些突变体在体外对巨噬细胞的杀伤也敏感。在不能产生超氧化物的J774巨噬细胞样细胞变异株中，重组突变体不再对巨噬细胞敏感。这表明沙门氏菌对DNA损伤的修复对于体内的完全毒力是必要的，并且吞噬细胞的氧化爆发是这种DNA损伤的一个来源。
Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage-like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative burst of phagocytes is one source of such DNA damage.