Effects of isoflurane anesthesia on the cardiovascular function of the C57BL/6 mouse.
Effects of isoflurane anesthesia on the cardiovascular function of the C57BL/6 mouse.
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DOI：
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发表时间：
2011
期刊：
影响因子：
2.5
通讯作者：
C. Constantinides;R. Mean;B. Janssen
中科院分区：
文献类型：
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作者：
C. Constantinides;R. Mean;B. Janssen
异氟醚(ISO)是用于小鼠实验干预的最常用的吸入麻醉剂，是需要小鼠保持相对较长时间麻醉的成像技术的首选。这项研究比较了平均动脉压(MAP)、心率(HR)和体温在ISO浓度为1%、1.5%和2%(体积比，v/v)下诱导后长达90分钟的稳定性。在所有三种麻醉水平下，我们检测了对氧气(FiO2)和一氧化二氮(N2O)吸气比变化的诱发生理反应。此外，我们还测定了麻醉对血液中pH、葡萄糖、胰岛素、糖皮质激素、血中氧分压和二氧化碳分压(PaO2、PaCO2)的影响。结果表明，最合适的ISO剂量水平是1.5%v/v，产生的MAP和HR值稳定，与动物清醒状态下观察到的值相当，MAP和HR的分钟变异性为0.11%。根据这样的记录，最佳的FiO2似乎是50%。增加N2O用量与MAP和HR的值更高和更稳定相关。动脉pH值在生理范围内，变化范围在7.20~7.43之间。1.5%v/v的ISO麻醉也与轻度高血糖(+47%)有关，而胰岛素水平和皮质类固醇水平保持不变。我们的结论是，异氟醚作为吸入麻醉剂在小鼠体内的应用可以通过以1.5%的v/v给药并补充N2O来优化以获得稳定的血流动力学。
Isoflurane (ISO) is the most commonly used inhalational anesthetic for experimental interventions in mice and is preferred for imaging technologies that require the mouse to remain anesthetized for relatively long time periods. This study compares the stability of mean arterial pressure (MAP), heart rate (HR), and body temperature under ISO concentrations of 1%, 1.5%, and 2% (volume-to-volume, v/v) for up to 90 minutes postinduction. At all three levels of anesthesia, we examined evoked physiological responses to fractional inspiratory ratio variations of oxygen (FiO2) and nitrous oxide (N2O). In addition, we determined the hemodynamic effects of anesthesia on pH, glucose, insulin, glucocorticoids, and partial pressure of oxygen and of carbon dioxide in the blood (paO2, paCO2). The results indicate that the most appropriate ISO dose level was 1.5% v/v, yielding stable MAP and HR values comparable to those observed in the animal's conscious state, with a minute-to-minute variability in MAP and HR of .11%. Based on such recordings, the optimal FiO2 appeared to be 50%. The additional use of N2O was associated with higher and more stable values of MAP and HR. Arterial pH values were within the physiological range and varied between 7.20 and 7.43. ISO anesthesia at 1.5% v/v was also associated with mild hyperglycemia (+47%), whereas insulin levels and corticosteroids remained unaltered. We conclude that the application of isoflurane as an inhalational anesthetic in the mouse can be optimized to attain stable hemodynamics by administering it at 1.5% v/v and by supplementing it with N2O.