LARGE-SCALE PRODUCTION OF VIBRIO-CHOLERAE TOXIN-B SUBUNIT FOR USE IN ORAL VACCINES
LARGE-SCALE PRODUCTION OF VIBRIO-CHOLERAE TOXIN-B SUBUNIT FOR USE IN ORAL VACCINES
复制标题
DOI：
10.1038/nbt1293-1574
复制
发表时间：
1993-12-01
期刊：
影响因子：
--
通讯作者：
HOLMGREN, J
中科院分区：
文献类型：
--
作者：
LEBENS, M;JOHANSSON, S;HOLMGREN, J
通过系统地操纵启动子和核糖体结合结构、质粒拷贝数和霍乱毒素B （CTB）亚基基因的结构，我们能够开发出一种质粒表达系统，当与优化的生长培养基结合使用时，可以提供接近每升1克的CTB产量。CTB蛋白的分泌率高达95%，可以很容易地从霍乱弧菌生产菌株的生长培养基中纯化出来，并且在免疫学上与以前使用的天然或重组CTB疫苗制剂没有区别。
By systematically manipulating promoter and ribosome binding structures, plasmid copy number and the structure of the cholera toxin B (CTB) subunit gene, we were able to develop a plasmid expression system that, when used in conjunction with an optimized growth medium, provided yields of CTB approaching one gram per liter. The CTB protein which was secreted to > 95%, could readily be purified from the growth medium of a V cholerae production strain 'and was shown to be immunologically indistinguishable from previously used vaccine preparations of native or recombinant CTB.