Pyramiding of insect- and disease-resistance genes into an elite indica, cytoplasm male sterile restorer line of rice, 'Minghui 63'
Pyramiding of insect- and disease-resistance genes into an elite indica, cytoplasm male sterile restorer line of rice, 'Minghui 63'
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DOI：
10.1046/j.1439-0523.2003.00917.x
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发表时间：
2004-04-01
期刊：
影响因子：
2
通讯作者：
Zhang, QF
中科院分区：
文献类型：
--
作者：
Jiang, GH;Xu, CG;Zhang, QF
将抗白叶枯病的野生稻显性基因Xa 21和抗鳞翅目昆虫的Bt融合基因cry 1Ab/cry 1Ac分别导入籼型细胞质雄性不育恢复系明恢63的同一遗传背景中。该品系表现出理想的抗虫和抗病表型。为了使这两个基因的效应最大化，还利用分子标记辅助选择的方法将这两个基因导入同一受体植株‘明恢63’中。在经历了卷叶螟和黄螟的自然侵染和接种Xoo菌株混合物后，该抗虫品系及其衍生的杂交种表现出高水平的抗虫害和病害。田间试验结果表明，该不育系与不育系珍汕97 A和马协A杂交，在不喷施化学药剂的条件下，其杂种的产量保持在相近水平，表明该不育系对受体系及其杂种具有一定的稳产作用。
The wild-rice-derived dominant gene Xa21 conferring multi-race resistance to bacterial blight and a fused Bt gene cry1Ab/cry1Ac conferring resistance to lepidopteran insects were individually introduced into the same genetic background of an elite indica cytoplasm male sterile (CMS) restorer line 'Minghui 63'. The line showed the desirable insect- and disease-resistant phenotypes. To maximize the effect, the two genes were also pyramided into the same recipient plant of 'Minghui 63' by marker-assisted selection. After being subjected to natural infestation of leaf-folders and yellow stem borers and inoculation of Xoo strain mixtures, the pyramiding line and its derived hybrids showed high levels of resistance against both insect damage and disease. Furthermore, data from field trials demonstrated that the hybrids made by crossing this pyramiding line with the CMS lines 'Zhenshan 97A' and 'Maxie A' retained a similar level of yield under conditions without chemical spray, indicating that the pyramiding genes have a yield-stabilizing effect on the recipient line and its hybrids.