ACh release from horse airway cholinergic nerves: effects of stimulation intensity and muscle preload.
ACh release from horse airway cholinergic nerves: effects of stimulation intensity and muscle preload.
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马气道胆碱能神经的乙酰胆碱释放：刺激强度和肌肉预负荷的影响。
DOI：
10.1152/ajplung.1993.264.3.l269
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Yu,M
中科院分区：
文献类型：
--
作者：
Wang,Z;Robinson,NE;Yu,M
本研究旨在确定刺激参数和肌肉预负荷对马气道胆碱能神经电场刺激 (EFS) 诱导的乙酰胆碱 (ACh) 释放的影响。制备气管条带束并悬浮在2ml组织浴中。刺激组织三到五次，每次 30 分钟。增加频率（0.5-16 Hz）和电压（5-20 V）会增加乙酰胆碱的释放；增加脉冲持续时间（0.5-3 ms）仅产生很小的影响。肌肉预负荷的改变（2-20 g）对乙酰胆碱释放没有影响。使用相同的 EFS 参数，ACh 释放在最多五个重复刺激周期内相当稳定。如果刺激量以皮摩尔每克每分钟表示，则刺激 15 分钟的组织释放的 ACh 量与 30 分钟相同，表明在 30 分钟的刺激期间，ACh 释放速率是恒定的。阿托品 (10(-6) M) 使乙酰胆碱的释放增强四到五倍，可能是通过消除乙酰胆碱对胆碱能神经的自身抑制作用。河豚毒素 (10(-6) M) 消除了 EFS 诱导的 ACh 释放。
This study was conducted to determine the effects of stimulation parameters and muscle preload on acetylcholine (ACh) release induced by electrical field stimulation (EFS) of horse airway cholinergic nerves. Trachealis strip bundles were prepared and suspended in 2-ml tissue baths. The tissues were stimulated three to five times for 30 min each. Increasing frequency (0.5-16 Hz) and voltage (5-20 V) increased ACh release; increasing pulse duration (0.5-3 ms) had only a minor effect. Alterations in muscle preload (2-20 g) had no effect on ACh release. ACh release was fairly constant for up to five repeated stimulation periods with the same EFS parameters. Stimulation of the tissues for 15 min released the same amount of ACh as 30 min if the amount was expressed as picomoles per gram per minute, suggesting that ACh release rate was constant during the 30-min period of stimulation. Atropine (10(-6) M) potentiated the release of ACh four- to fivefold, presumably by removing the autoinhibitory effect of ACh on the cholinergic nerves. Tetrodotoxin (10(-6) M) abolished the EFS-induced ACh release.