Dynamic positional fate map of the primary heart-forming region
Dynamic positional fate map of the primary heart-forming region
复制标题
DOI：
10.1016/j.ydbio.2009.05.570
复制
发表时间：
2009-08-15
影响因子：
2.7
通讯作者：
Rongish, Brenda J.
中科院分区：
文献类型：
--
作者：
Cui, Cheng;Cheuvront, Tracey J.;Rongish, Brenda J.
在这里，我们显示了在细胞水平分辨率的早期心脏形态发生的时空编排，在体内，并调和冲突的位置命运映射数据有关的主要心脏形成领域（S）。我们使用精密电穿孔方法结合宽视野延时显微镜在鹌鹑胚胎（一种温血脊椎动物（HH阶段4至10））中确定心前细胞的位置命运。与先前的研究相反，结果表明存在跨越中线的“连续”圆形心脏场，出现在HH阶段4，然后在HH阶段5-7扩展形成宽弧的祖细胞。我们的时间分辨图像数据显示，这些心脏祖细胞的一个亚组与常见的心源性因子Nkx-2.5和Bmp-2的表达不重叠，直到HH阶段10，当管状心脏形成时，这就引发了心脏命运何时被指定以及哪些关键因素的问题。心脏前体细胞的亚群和解剖带（队列）在很大程度上由内胚层折叠和其他大规模组织变形驱动的过程中显着改变其相对位置。因此，我们的新的动态位置命运图解决了心脏祖细胞的起源，在Alabotes。这些数据还确立了组织运动对细胞位置命运有显著贡献的概念，即，在中线心管组装（HH第9阶段）期间发生的大部分细胞移位不是由于“迁移”（自主运动），这是一种普遍持有的信念。对我们时间分辨数据的计算分析为更精确地分析心脏基因调控网络如何与鸟类和哺乳动物的早期心脏组织形态发生相关奠定了基础。(C)2009 Elsevier Inc. All rights reserved.
Here we show the temporal-spatial orchestration of early heart morphogenesis at cellular level resolution, in vivo, and reconcile conflicting positional fate mapping data regarding the primary heart-forming field(s). We determined the positional fates of precardiac cells using a precision electroporation approach in combination with wide-field time-lapse microscopy in the quail embryo, a warm-blooded vertebrate (HH Stages 4 through 10). Contrary to previous studies, the results demonstrate the existence of a "continuous" circle-shaped heart field that spans the midline, appearing at HH Stage 4, which then expands to form a wide arc of progenitors at HH Stages 5-7. Our time-resolved image data show that a Subset of these cardiac progenitor cells do not overlap with the expression of common cardiogenic factors, Nkx-2.5 and Bmp-2, until HH Stage 10, when a tubular heart has formed, calling into question when cardiac fate is specified and by which key factors. Sub-groups and anatomical bands (cohorts) of heart precursor cells dramatically change their relative positions in a process largely driven by endodermal folding and other large-scale tissue deformations. Thus, our novel dynamic positional fate maps resolve the origin of cardiac progenitor cells in amniotes. The data also establish the concept that tissue motion contributes significantly to cellular position fate - i.e., much of the cellular displacement that occurs during assembly of a midline heart tube (HH Stage 9) is NOT due to "migration" (autonomous motility), a commonly held belief. Computational analysis of our time-resolved data lays the foundation for more precise analyses of how cardiac gene regulatory networks correlate with early heart tissue morphogenesis in birds and mammals. (C) 2009 Elsevier Inc. All rights reserved.