Novel hypnotics of Japanese traditional herbal medicines to caffeine-induced insomnia in Drosophila by using Newly-developed automated sleep and rhythm analysis system (AutoCircaS)
Novel hypnotics of Japanese traditional herbal medicines to caffeine-induced insomnia in Drosophila by using Newly-developed automated sleep and rhythm analysis system (AutoCircaS)
复制标题
DOI：
10.1016/j.gene.2022.146852
复制
发表时间：
2022-09-11
期刊：
影响因子：
3.5
通讯作者：
Ishida, Norio
中科院分区：
文献类型：
--
作者：
Inoue, Eiji;Suzuki, Takahiro;Ishida, Norio
果蝇的睡眠是在2000年由果蝇活动监测系统（DAM）定义的。但DAM的管道空间非常小，每个管道一只苍蝇对于分析苍蝇的社会行为来说非常有限。为了克服DAM系统的这些缺点，我们开发了一种新的自动睡眠和节律分析系统（AutoCircaS），该系统使用延时（每10秒一帧）成像来监测和记录自由空间中的果蝇（Drosophila）和小鱼青鳉（Oryzias latipes）的任何行为，如社会交配、睡眠和昼夜节律。AutoCircaS可以检测咖啡因诱导的果蝇失眠在光暗（LD）和恒定黑暗（DD）条件下。因此，使用AutoCircaS，我们发现日本传统草药KyushinKannouGan-ki（KKG），NouKassei（NK）以及Sansoninto显着改善了咖啡因引起的苍蝇失眠。数据表明，AutoCircaS对于小动物的睡眠分析和筛选多种来源的新型镇静催眠药非常有用。
Sleep in Drosophila was defined in the year 2000 by using Drosophila Activity Monitor (DAM) system. But DAM is very small tube space and one fly per tube is very limited to analyze for fly social behavior. To overcome such demerits of DAM system, we developed a novel automated sleep and rhythm analysis system (AutoCircaS) which monitors and records any behaviors like social mating, sleep, and circadian rhythm in flies (Drosophila) and small fishes medaka (Oryzias latipes) in free space using the time-lapse (one frame per 10 sec) imaging. AutoCircaS can detect the caffeine-induced insomnia in flies in light-dark (LD) and constant dark (DD) conditions. Thus, using the AutoCircaS, we discovered that Japanese traditional herbal medicines, KyushinKannouGan-ki (KKG), NouKassei (NK) as well as, and Sansoninto, significantly improved caffeine-induced insomnia in flies. The data suggest that AutoCircaS is useful for sleep analysis of small animals and screening of new sedative-hypnotics from many origins.