STRUCTURAL VARIANTS OF HUMAN T200 GLYCOPROTEIN (LEUKOCYTE-COMMON ANTIGEN)
STRUCTURAL VARIANTS OF HUMAN T200 GLYCOPROTEIN (LEUKOCYTE-COMMON ANTIGEN)
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DOI：
10.1002/j.1460-2075.1987.tb02361.x
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发表时间：
1987-05-01
期刊：
影响因子：
11.4
通讯作者：
TROWBRIDGE, IS
中科院分区：
文献类型：
--
作者：
RALPH, SJ;THOMAS, ML;TROWBRIDGE, IS
已检测到人T200糖蛋白一级结构的结构变异。已鉴定了三种cDNA变异体，由于其氨基末端区域的序列差异，每种变异体都编码大小不同的T200分子。分子的最大形式与最小形式的区别在于，在前八个氨基末端残基之后插入了161个氨基酸。另一个变异体在同一位置有一个插入物，其47个氨基酸与较大插入物中的75-121位氨基酸相同。这两个额外的结构域都富含丝氨酸和苏氨酸残基，并可能显示多个O-连接的寡糖。这些可能由细胞类型特异性选择性剪接引起的结构变异为先前观察到的T200糖蛋白的结构和抗原异质性提供了分子基础。人T200糖蛋白的外区除氨基酸末端可变区外，还包括一个约400个氨基酸的富含半胱氨酸的第二区、一个跨膜区和一个由707个氨基酸组成的大的细胞质结构域，所有结构变体都共享该区域，并且在物种间高度保守。编码人类T200的基因位于1号染色体的长臂上。
Structural variation in the primary structure of human T200 glycoprotein has been detected. Three cDNA variants have been characterized each of which encode T200 molecules that differ in size as a result of sequence differences in their amino-terminal regions. The largest form of the molecule is distinguished from the smallest by an insert of 161 amino acids, after the first eight amino-terminal residues. The other variant has an insert at the same location of 47 amino acids identical to residues 75-121 in the larger insert. Both extra domains are rich in serine and threonine residues and are likely to display multiple O-linked oligosaccharides. These structural variants which probably arise by cell-type-specific alternative splicing provide a molecular basis for the previously observed structural and antigenic heterogeneity of T200 glycoprotein. In addition to the variable amino-terminal region, the external domain of human T200 glycoprotein consists of a second cysteine-rich region of about 400 amino acids, a single transmembrane-spanning region and a large cytoplasmic domain of 707 amino acids shared by all of the structural variants and highly conserved between species. The gene encoding human t200 is located on the long arm of chromosome 1.