3D Printed Silicone-Hydrogel Scaffold with Enhanced Physicochemical Properties
3D Printed Silicone-Hydrogel Scaffold with Enhanced Physicochemical Properties
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DOI：
10.1021/acs.biomac.5b01722
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发表时间：
2016-04-01
影响因子：
6.2
通讯作者：
Emneus, Jenny
中科院分区：
文献类型：
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作者：
Mohanty, Soumyaranjan;Alm, Martin;Emneus, Jenny
具有多种功能的支架在组织工程领域引起了广泛的关注，因为它们能够通过各种线索（包括机械、化学和电学）来控制细胞行为。由临床批准的材料制造这种支架目前是一个巨大的挑战。这项工作的目标是从临床批准的材料制造组织工程支架，具有递送生物分子和直接细胞命运的能力。我们使用了一种简单的3D打印方法，将聚合物浇铸与超临界流体技术相结合，以生产有机硅-聚（甲基丙烯酸2-羟乙酯）-共-聚（乙二醇）甲基醚丙烯酸酯（pHEMA-co-PEGMEA）的3D互穿聚合物网络（IPN）支架。采用pHEMA-co-PEGMEA IPN材料来支持人间充质干细胞（hMSC）的生长，从而在3周的时间内产生高细胞活力和代谢活性。此外，IPN支架支持多孔支架内的3D组织形成，在支架表面上具有良好散布的细胞形态。作为概念的证明，证明了从pHEMA-co-PEGMEA IPN持续释放强力霉素（DOX），并且使用HeLa细胞的DOX调节的绿色荧光报告基因（GFP）表达测定证实了从IPN释放的药物的生物活性。鉴于其独特的机械和药物释放特征，IPN支架可通过从其水凝胶网络中释放各种化学物质来指导干细胞分化。
Scaffolds with multiple functionalities have attracted widespread attention in the field of tissue engineering due to their ability to control cell behavior through various cues, including mechanical, chemical, and electrical. Fabrication of such scaffolds from clinically approved materials is currently a huge challenge. The goal of this work was to fabricate a tissue engineering scaffold from clinically approved materials with the capability of delivering biomolecules and direct cell fate. We have used a simple 3D printing approach, that combines polymer casting with supercritical fluid technology to produce 3D interpenetrating polymer network (IPN) scaffold of silicone-poly(2-hydroxyethyl methacrylate)-co-poly(ethylene glycol) methyl ether acrylate (pHEMA-co-PEGMEA). The pHEMA-co-PEGMEA IPN materials were employed to support growth of human mesenchymal stem cells (hMSC), resulting in high cell viability and metabolic activity over a 3 weeks period. In addition, the IPN scaffolds support 3D tissue formation inside the porous scaffold with well spread cell morphology on the surface of the scaffold. As a proof of concept, sustained doxycycline (DOX) release from pHEMA-co-PEGMEA IPN was demonstrated and the biological activity of released drug from IPN was confirmed using a DOX regulated green fluorescent reporter (GFP) gene expression assay with HeLa cells. Given its unique mechanical and drug releasing characteristics, IPN scaffolds may be used for directing stem cell differentiation by releasing various chemicals from its hydrogel network.