Intrapulse temporality between pulses of a metabolite of prostaglandin F2α and circulating concentrations of progesterone before, during, and after spontaneous luteolysis in heifers
Intrapulse temporality between pulses of a metabolite of prostaglandin F2α and circulating concentrations of progesterone before, during, and after spontaneous luteolysis in heifers
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DOI：
10.1016/j.theriogenology.2010.05.018
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发表时间：
2010-10-15
期刊：
影响因子：
2.8
通讯作者：
Beg, M. A.
中科院分区：
文献类型：
--
作者：
Ginther, O. J.;Shrestha, H. K.;Beg, M. A.
在7头小母牛的黄体溶解前、黄体溶解和黄体溶解后期间，每小时测定前列腺素F-2 α（PGF）代谢产物13，14-二氢-15-酮-PGF（2 α）（PGFM）的脉冲和孕酮的脉冲内浓度。当仅在连续振荡的峰值处进行评估时，黄体溶解前结束和黄体溶解开始的常见时间是基于黄体酮的进行性减少。黄体溶解期结束定义为孕酮降低至1 ng/mL。从排卵后第15天开始每小时采集血样，直至通过彩色多普勒超声检查确定黄体消退。在黄体溶解前期最后一次PGFM脉冲的第-2小时和第2小时（第0小时= PGFM峰值）之间，黄体酮在第-1小时和第0小时之间下降，然后恢复到脉冲前浓度。此后浓度无显著变化，直至黄体溶解期早期的PGFM脉冲;孕酮在0小时下降，并在0小时后短暂反弹，但未达到脉冲前浓度。在黄体溶解期的后期，孕酮也在第-1小时和第0小时之间下降，但没有反弹。黄体溶解结束后，孕酮在PGFM脉冲中略有下降。结果表明，第一次在PGFM脉冲孕酮浓度的模式之间的preluteolytic，luteolytic，和postluteolytic期间有很大的不同。(C)2010年爱思唯尔公司All rights reserved.
Pulses of the prostaglandin F-2 alpha, (PGF) metabolite 13,14-dihydro-15-keto-PGF(2 alpha), (PGFM) and the intrapulse concentrations of progesterone were characterized hourly during the preluteolytic, luteolytic, and postluteolytic periods in seven heifers. The common hour of the end of preluteolysis and the beginning of luteolysis was based on a progressive progesterone decrease when assessed only at the peaks of successive oscillations. The end of the luteolytic period was defined as a decrease in progesterone to 1 ng/mL. Blood samples were taken hourly from 15 d after ovulation until luteal regression as determined by color-Doppler ultrasonography. Between Hours -2 and 2 (Hour 0 = PGFM peak) of the last PGFM pulse of the preluteolytic period, progesterone decreased between Hours -1 and 0, and then returned to the prepulse concentration. Concentration did not change significantly thereafter until a PGFM pulse early in the luteolytic period; progesterone decreased by Hour 0 and transiently rebounded after Hour 0, but not to the prepulse concentration. In the later portion of the luteolytic period, progesterone also decreased between Hours -1 and 0 but did not rebound. After the defined end of luteolysis, progesterone decreased slightly throughout a PGFM pulse. Results demonstrated for the first time that the patterns of progesterone concentrations within a PGFM pulse differ considerably among the preluteolytic, luteolytic, and postluteolytic periods. (C) 2010 Elsevier Inc. All rights reserved.