Relationship between β-oxidation pathway and the hydrocarbon-degrading profile in actinomycetes bacteria
Relationship between β-oxidation pathway and the hydrocarbon-degrading profile in actinomycetes bacteria
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DOI：
10.1016/s0964-8305(02)00120-8
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发表时间：
2003-01-01
影响因子：
4.8
通讯作者：
Alvarez, HM
中科院分区：
文献类型：
--
作者：
Alvarez, HM
对红球菌属、诺卡氏菌属、戈登氏菌属和迪茨氏菌属的9株非产孢土壤放线菌的烃类降解能力进行了研究。所有菌株显示出相似的烃利用概况。正构和支链烷烃、烷基苯和烷基环烷烃比单芳烃、多芳烃和环烷烃更容易被用作碳源。因此，所研究的菌株对多种烃类表现出更大的亲和力，这些烃类被引导到β-氧化途径进行降解;例如十五烷、十六烷、2，6，10，14-四甲基十五烷（姥鲛烷）、苯基癸烷、瓦斯油和煤油。此外，这些碳氢化合物被利用的生物合成和积累的三酰基甘油的研究细菌在氮限制条件下培养。本研究的结果表明，β-氧化途径，单独或紧密耦合到从头脂肪酸生物合成途径，作为脂肪酸的主要来源，用于在烃上的细胞生长过程中的脂质生物合成，这支持了良好的细胞生长。非产孢放线菌的代谢可能适应于利用其结构中具有烷基侧链的烃类来生产具有不同复杂性和功能的大量种类的脂质。在其化学结构中具有烷基侧链的烃类的降解，以及放线菌在自然界中经常发生的条件下转化细胞脂质中的此类烃类的能力，使这些微生物成为污染环境中生物修复活动的潜在候选者。(C)2002爱思唯尔科技有限公司版权所有。
Nine non-sporulating soil actinomycetes bacteria belonging to Rhodococcus, Nocardia, Gordonia and Dietzia genera were investigated for their hydrocarbon-degrading abilities. All strains showed similar hydrocarbon-utilizing profiles. Normal- and branched-alkanes, alkylbenzenes and alkylcycloalkanes were utilized as carbon sources more readily than monoaromatics, polyaromatics and cycloalkanes. Thus, the studied strains showed more affinity for a variety of hydrocarbons, which were channelled to beta-oxidation pathway for degradation; such as pentadecane, hexadecane, 2,6,10,14-tetramethyl pentadecane (pristane), phenyldecane, gas-oil and kerosene. In addition, these hydrocarbons were utilized for the biosynthesis and accumulation of triacylglycerols by the studied bacteria during cultivation under nitrogen-limiting conditions. The results of this study showed that beta-oxidation pathway, solely or tightly coupled to the de novo fatty acid biosynthesis route, serves as the main source of fatty acids for lipid biosynthesis during growth of the cells on hydrocarbons, which supported good growth of cells. The metabolism of non-sporulating actinomycetes may adapt to the utilization of hydrocarbons with an alkyl side chain in their structure for the production of a large variety of lipids with different complexity and functions.The degradation of hydrocarbons containing an alkyl side chain in their chemical structure, as well as the capability of actinomycetes for transforming such hydrocarbons in cellular lipids under conditions that occur often in the nature, make these microorganisms potential candidates for bioremediation activities in contaminated environments. (C) 2002 Elsevier Science Ltd. All rights reserved.