THERMODYNAMIC STUDIES OF BASE-PAIRING INVOLVING 2,6-DIAMINOPURINE
THERMODYNAMIC STUDIES OF BASE-PAIRING INVOLVING 2,6-DIAMINOPURINE
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DOI：
10.1093/nar/16.11.5115
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发表时间：
1988-06-10
影响因子：
14.9
通讯作者：
CHOLLET, A
中科院分区：
文献类型：
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作者：
CHEONG, C;TINOCO, I;CHOLLET, A
用光学熔融法测定了含有2，6-二氨基嘌呤(D)的寡核苷酸双链与四种正常DNA碱基匹配的热稳定性。光学熔化曲线的比较得出了在其他相同的碱基对序列中含D的标准碱基对的相对稳定性。在dC3DG3：dC3TG3中，D：T对比A：T对更稳定，在dCT3DT3G：dCA3TA3G中，D：T对与A：T对一样稳定，而在dCA3DA3G：dCT7G中，D：T对比A：T对更稳定。DNA双链dCA3XA3G：dCT3YT3G中X：Y的稳定性顺序为：(A：T)>；(T：D).simeq。(D：T).gtoreq.(T：A)>；(C：D).simeq.(D：G).gtoreq.(D：C)(G：D).simeq.(D：D).gtoreq.(A：D)。
The thermal stabilities of oligodeoxyribonucleotide duplexes containing 2,6-diaminopurine (D) matched with each of the four normal DNA bases were determined by optical melting techniques. Comparison of optical melting curves yielded relative stabilities for the D-containing standard base pairs in an otherwise identical base-pair sequence. The D:T pair was found to be more stable than the A:T pair in dC3DG3:dC3TG3, as stable as the A:T in dCT3DT3G:dCA3TA3G, and less stable than the A:T in dCA3DA3G:dCT7G. The order of stabilities for X:Y in the DNA duplex dCA3XA3G:dCT3YT3G is: (A:T) > (T:D) .simeq. (D:T) .gtoreq. (T:A) > (C:D) .simeq. (D:G) .gtoreq. (D:C) (G:D) .simeq. (D:D) .gtoreq. (A:D).