Characterization of PHB1 and its role in mitochondrial maturation and yolk platelet degradation during development of Artemia embryos.
Characterization of PHB1 and its role in mitochondrial maturation and yolk platelet degradation during development of Artemia embryos.
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DOI：
10.1371/journal.pone.0109152
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yang WJ
中科院分区：
文献类型：
--
作者：
Ye X;Zhao Y;Zhao LL;Sun YX;Yang JS;Yang WJ
为了应对恶劣的环境，卤虫等甲壳类动物会产生新陈代谢受到抑制的滞育原肠胚胚胎（囊肿）。滞育后发育期间代谢和发育恢复，但这些细胞事件背后的机制仍然很大程度上未知。我们的研究调查了抑制素 1 (PHB1) 在滞育后发育过程中代谢重新启动中的作用。我们发现 PHB1 通过磷酸化状态和定位的变化进行发育调节。 RNA 干扰实验的结果表明，PHB1 对于发育过程中的线粒体成熟和卵黄降解至关重要。此外，PHB1存在于卵黄血小板中，并且在卵黄蛋白的蛋白水解过程中经历泛素介导的降解。 PHB1 在丰年虫发育过程中协调线粒体成熟和卵黄血小板降解中起着不可或缺的作用。 PHB1 的这一新功能为理解 PHB1 在代谢和发育中的作用提供了新的线索。
To cope with harsh environments, crustaceans such as Artemia produce diapause gastrula embryos (cysts) with suppressed metabolism. Metabolism and development resume during post-diapause development, but the mechanism behind these cellular events remains largely unknown. Our study investigated the role of prohibitin 1 (PHB1) in metabolic reinitiation during post-diapause development. We found that PHB1 was developmentally regulated via changes in phosphorylation status and localization. Results from RNA interference experiments demonstrated PHB1 to be critical for mitochondrial maturation and yolk degradation during development. In addition, PHB1 was present in yolk platelets, and it underwent ubiquitin-mediated degradation during the proteolysis of yolk protein. PHB1 has an indispensable role in coordinating mitochondrial maturation and yolk platelet degradation during development in Artemia. This novel function of PHB1 provides new clues to comprehend the roles of PHB1 in metabolism and development.
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影响因子：
--
作者：
Polier, Gemot;Neumann, Jennifer;Li-Weber, Min
通讯作者：
Li-Weber, Min
DOI：
10.1016/j.bbamcr.2009.05.008
发表时间：
2009-08-01
影响因子：
5.1
作者：
Ande, Sudharsana R.;Gu, Yuanyuan;Mishra, Suresh
通讯作者：
Mishra, Suresh
影响因子：
64.8
作者：
Artal-Sanz, Marta;Tavernarakis, Nektarios
通讯作者：
Tavernarakis, Nektarios
影响因子：
9.2
作者：
Coates, PJ;Jamieson, DJ;Hall, PA
通讯作者：
Hall, PA
影响因子：
4.9
作者：
Ande, S. R.;Xu, Z.;Mishra, S.
通讯作者：
Mishra, S.