In Vitro Exposure to Increasing Micafungin Concentrations Easily Promotes Echinocandin Resistance in Candida glabrata Isolates
In Vitro Exposure to Increasing Micafungin Concentrations Easily Promotes Echinocandin Resistance in Candida glabrata Isolates
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DOI：
10.1128/aac.01542-16
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发表时间：
2017-02-01
影响因子：
4.9
通讯作者：
Guinea, Jesus
中科院分区：
文献类型：
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作者：
Angeles Bordallo-Cardona, Maria;Escribano, Pilar;Guinea, Jesus
我们评估了5株棘白菌素敏感的光秃假丝酵母暴露于米卡芬金后的体外敏感性。不同浓度的米卡芬菌素直接暴露于平板上，对生长的抑制作用为0.062 μ g/ml。用0.031 μ g/ml的米卡芬菌素在板上逐级曝光，在浓度为下2倍的板上依次繁殖；micafungin和anidulafungin的mic依次升高，所有分离株均出现棘白菌素耐药，fks2突变。
We assessed the in vitro susceptibility of five echinocandin-susceptible Candida glabrata isolates after exposure to micafungin. The direct exposure to plates at different micafungin concentrations resulted in the inhibition of growth at 0.062 mu g/ml. The progressive exposure was performed on plates using 0.031 mu g/ml of micafungin and sequential propagation on plates containing the next 2-fold concentration; the MICs of micafungin and anidulafungin increased sequentially, and all the isolates became echinocandin resistant, showing fks2 mutations.