In vitro selection identifies key determinants for loop-loop interactions:: RNA aptamers selective for the TAR RNA element of HIV-1
In vitro selection identifies key determinants for loop-loop interactions:: RNA aptamers selective for the TAR RNA element of HIV-1
复制标题
DOI：
10.1017/s1355838299991318
复制
发表时间：
1999-12-01
期刊：
影响因子：
4.5
通讯作者：
Toulmé, JJ
中科院分区：
文献类型：
--
作者：
Ducongé, F;Toulmé, JJ
We selected RNA aptamers specific for the trans-activation responsive (TAR) RNA, a stem-loop structure crucial for the transcription of the integrated genome of the human immunodeficiency virus, Most of the selected sequences could be folded as imperfect hairpins and displayed a 5'-GUCCCAGA-3' consensus motif constituting the apical loop, The six central bases of this consensus sequence are complementary to the entire TAR loop, leading to the formation of TAR RNA-aptamer "kissing" complexes. The consensus G and A residues dosing the aptamer loop contributed to the high affinity (K-d = 30 nM at 23 degrees C) of the aptamers for the TAR RNA, This G.A pair was shown to be crucial for binding to TAR at a low magnesium concentration, The selection also identified 5'-PuPy and 5'-PyPu base pairs at alpha and beta positions of the stem, next to the loop, respectively, This strategy offered a way to identify key determinants of loop-loop interactions and to generate high affinity ligands of TAR RNA structure.
