Protease treatment affects both invasion ability and biofilm formation in Listeria monocytogenes
Protease treatment affects both invasion ability and biofilm formation in Listeria monocytogenes
复制标题
DOI：
10.1016/j.micpath.2008.01.007
复制
发表时间：
2008-07-01
影响因子：
3.8
通讯作者：
Selan, Laura
中科院分区：
文献类型：
--
作者：
Longhi, Catia;Scoarughi, Gian Luca;Selan, Laura
单核细胞增多性李斯特菌是一种显著的侵袭性细菌，与人类威胁生命的食源性疾病有关。一些表面蛋白已被证明在单核细胞增多性李斯特菌的黏附和随后的吞噬细胞入侵中是必不可少的。由于李斯特菌对宿主细胞入侵的控制可能会阻碍其在感染宿主中的传播，我们检测了蛋白酶处理对L单核细胞形成生物膜和入侵组织能力的影响。我们选择了粘质沙雷氏菌产生的一种胞外金属蛋白酶(SPEP)，它已经被广泛用作抗炎剂，并已被证明在其他细菌中调节粘附素的表达和诱导抗生素敏感性。用亚致死浓度的SPEP处理单核细胞增生性李斯特菌，降低了它们形成生物膜和入侵宿主细胞的能力。酶谱显示Ami4b自溶素、内毒素B和ActA显著减少。这些细胞表面蛋白在这些细菌和它们的宿主细胞之间的相互作用中起到了配基的作用，我们的数据表明，用这种天然的酶处理可能会提供一个有用的工具来防止L单核细胞增多症与人类肠道的初始黏附。(C)2008年，爱思唯尔有限公司出版。
Listeria monocytogenes is a notably invasive bacterium associated with life-threatening food-borne disease in humans. Several surface proteins have been shown to be essential in the adhesion of L. monocytogenes, and in the subsequent invasion of phagocytes. Because the control of the invasion of host cells by Listeria could potentially hinder its spread in the infected host, we have examined the effects of a protease treatment on the ability of L monocytogenes to form biofilms and to invade tissues. We have chosen serratiopeptidase (SPEP), an extracellular metalloprotease produced by Serratia marcescens that is already widely used as an anti-inflammatory agent, and has been shown to modulate adhesin expression and to induce antibiotic sensitivity in other bacteria. Treatment of L. monocytogenes with sublethal concentrations of SPEP reduced their ability to form biofilms and to invade host cells. Zymograms of the treated cells revealed that Ami4b autolysin, internalinB, and ActA were sharply reduced. These cell-surface proteins are known to function as ligands in the interaction between these bacteria and their host cells, and our data suggest that treatment with this natural enzyme may provide a useful tool in the prevention of the initial adhesion of L monocytogenes to the human gut. (c) 2008 Published by Elsevier Ltd.