Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13
Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13
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DOI：
10.1101/gad.11.4.512
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发表时间：
1997-02-15
影响因子：
10.5
通讯作者：
Charbonneau, M
中科院分区：
文献类型：
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作者：
Grandin, N;Reed, SI;Charbonneau, M
我们已经分离了STN 1，一个重要的酿酒酵母基因，作为cdc 13 -1突变的抑制因子。观察到温度敏感的突变型等位基因STN 1，stn 1 -13和cdc 13 -1之间的合成致死相互作用。Stn 1和Cdc 13蛋白显示了物理相互作用的双杂交分析。如前所述，cdc 13 -1，stn 1 -13细胞在限制性温度下积累单链DNA在染色体的亚端粒区域，但程度低于cdc 13 -1细胞。此外，Cdc 13和Stn 1都被发现参与端粒长度的调节，STN 1或Cdc 13中的突变赋予端粒大小的增加。Stn 1功能的丧失激活了RAD 9和MEC 3 G（2）/M检查点，因此证实了DNA损伤的产生。我们建议，Stn 1功能在端粒代谢后期S期与Cdc 13合作。
We have isolated STN1, an essential Saccharomyces cerevisiae gene, as a suppressor of the cdc13-1 mutation. A synthetic lethal interaction between a temperature-sensitive mutant allele of STN1, stn1-13, and cdc13-1 was observed. Stn1 and Cdc13 proteins displayed a physical interaction by two-hybrid analysis. As shown previously for cdc13-1, stn1-13 cells at the restrictive temperature accumulate single-stranded DNA in subtelomeric regions of the chromosomes, but to a lesser extent than cdc13-1 cells. In addition, both Cdc13 and Stn1 were found to be involved in the regulation of telomere length, mutations in STN1 or CDC13 conferring an increase in telomere size. Loss of Stn1 function activated the RAD9 and MEC3 G(2)/M checkpoints, therefore confirming that DNA damage is generated. We propose that Stn1 functions in telomere metabolism during late S phase in cooperation with Cdc13.