Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants
Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants
复制标题
植物中跨高尔基体网络囊泡的分离和糖组分析
DOI：
10.1007/978-1-0716-0767-1_13
复制
发表时间：
2020
影响因子：
--
通讯作者：
Guangxi Ren, Michel Ruiz
中科院分区：
文献类型：
--
作者：
Guangxi Ren, Michel Ruiz
动态内膜系统有助于分拣和运输各种货物。因此，它对植物的生长和发育至关重要。近年来囊泡蛋白质组学研究取得了实质性进展。相比之下，人们对介导多糖往返质膜转运的囊泡隔室的身份以及它们选择性转运的糖类型知之甚少。在这一章中，我们提供了一个详细的描述用于SYP61囊泡群体糖组的说明协议。我们的方法可以很容易地进行糖组学研究的各种植物细胞囊泡群体定义通过亚细胞标记或不同的治疗。
The dynamic endomembrane system facilitates sorting and transport of diverse cargo. Therefore, it is crucial for plant growth and development. Vesicle proteomic studies have made substantial progress in recent years. In contrast, much less is known about the identity of vesicle compartments that mediate the transport of polysaccharides to and from the plasma membrane and the types of sugars they selectively transport. In this chapter, we provide a detailed description of the protocol used for the elucidation of the SYP61 vesicle population glycome. Our methodology can be easily adapted to perform glycomic studies of a broad variety of plant cell vesicle populations defined via subcellular markers or different treatments.