Microcontact peeling: A cell micropatterning technique for circumventing direct adsorption of proteins to hydrophobic PDMS.
Microcontact peeling: A cell micropatterning technique for circumventing direct adsorption of proteins to hydrophobic PDMS.
复制标题
微接触剥离：一种细胞微图案化技术，用于避免蛋白质直接吸附到疏水性 PDMS。
DOI：
10.1002/cpcb.22
复制
发表时间：
2017
影响因子：
--
通讯作者：
S. Deguchi
中科院分区：
文献类型：
--
作者：
S. Yokoyama;T. S. Matsui;S. Deguchi
微接触印刷（μCPr）是用于细胞微图案化的最流行的技术之一。在传统的μCPr中，具有微特征的聚二甲基硅氧烷（PDMS）印模用于将细胞外基质（ECM）蛋白吸附到特征表面上，并将它们转移到细胞培养基质的特定区域。然而，已经报道了ECM以外的某些类型的功能蛋白质在直接吸附到疏水PDMS时变性。在这里，我们描述了一种替代技术的详细协议-微接触剥离（μCPe）-允许细胞微图案化，同时绕过将蛋白质吸附到裸PDMS的步骤。该技术采用具有相对高表面能的微特征材料（例如铜），而不是使用微特征PDMS印模，以剥离存在于基底表面上的细胞粘附层。因此，细胞非粘附性基底在经历与微特征材料的物理接触的特定表面处暴露。因此，尽管μCPe和μCPr表面上相似，但前者不包括通过疏水PDMS转移生物分子的过程。John Wiley & Sons，Inc.
Microcontact printing (μCPr) is one of the most popular techniques used for cell micropatterning. In conventional μCPr, a polydimethylsiloxane (PDMS) stamp with microfeatures is used to adsorb extracellular matrix (ECM) proteins onto the featured surface and transfer them onto particular areas of a cell culture substrate. However, some types of functional proteins other than ECM have been reported to denature upon direct adsorption to hydrophobic PDMS. Here we describe a detailed protocol of an alternative technique––microcontact peeling (μCPe)––that allows for cell micropatterning while circumventing the step of adsorbing proteins to bare PDMS. This technique employs microfeatured materials with a relatively high surface energy such as copper, instead of using a microfeatured PDMS stamp, to peel off a cell‐adhesive layer present on the surface of substrates. Consequently, cell‐nonadhesive substrates are exposed at the specific surface that undergoes the physical contact with the microfeatured material. Thus, although μCPe and μCPr are apparently similar, the former does not comprise a process of transferring biomolecules through hydrophobic PDMS. © 2017 by John Wiley & Sons, Inc.