MECHANISM OF ACTION OF A YEAST ACTIVATOR - DIRECT EFFECT OF GAL4 DERIVATIVES ON MAMMALIAN TFIID-PROMOTER INTERACTIONS
MECHANISM OF ACTION OF A YEAST ACTIVATOR - DIRECT EFFECT OF GAL4 DERIVATIVES ON MAMMALIAN TFIID-PROMOTER INTERACTIONS
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DOI：
10.1016/s0092-8674(88)80011-4
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发表时间：
1988-08-26
期刊：
影响因子：
64.5
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
--
作者：
HORIKOSHI, M;CAREY, MF;ROEDER, RG
我们分析了哺乳动物TATA因子（TFID）和酵母激活剂GAL 4的衍生物之间的相互作用。腺病毒E4启动子上的TATA因子与邻近TATA位点的GAL 4结合位点的相互作用因GAL 4结合而发生定性改变。观察到TFIID相互作用的改变与两个GAL4衍生物，刺激杂合E4启动子活性在体外，但没有与第三个衍生物，结合到DNA，但没有显示激活。这些结果表明，TFIID是一个GAL4激活域的直接目标，并提出了一个简单的一般模型的激活机制。
We have analyzed interactions between the mammalian TATA factor (TFID) and derivatives of the yeast activator GAL4. The interaction of the TATA factor on the adenovirus E4 promoter with GAL4 binding sites adjacent to the TATA site was qualitatively altered in response to GAL4 binding. Alterations in the TFIID interactions were observed with two GAL4 derivatives that stimulated hybrid E4 promoter activity in vitro but not with a third derivative that bound to DNA but showed no activation. These results indicate that TFIID is a direct target of a GAL4 activation domain and suggest a simple general model for the activation mechanism.