Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B.
Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B.
复制标题
大肠杆菌B的Na/谷氨酸同向转运载体基因gltS的核苷酸序列。
DOI：
10.1016/s0021-9258(18)45797-3
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Y. Anraku
中科院分区：
文献类型：
--
作者：
Y. Deguchi;Ichiro Yamato;Y. Anraku
测定了大肠杆菌B Na+/谷氨酸共转运载体gltS基因的核苷酸序列，并推导了该载体蛋白的氨基酸序列。预测的谷氨酸载体由401个氨基酸组成，分子量为42，455。在gltS基因的5 '侧翼区发现了Shine-Dalgarno序列和推定的启动子序列。预测的蛋白质是非常疏水的（73%的非极性氨基酸），并从其亲水性的配置文件，该蛋白质是由12个疏水跨膜段，平均长度为21.6个残基/段。在gltS基因下游发现了一个典型的非rho依赖性转录终止信号。我们发现了5个氨基酸残基的保守序列（Gly 42-Ala 82-X-X-X-X-Leu 87-X-X-X-Gly 91-Arg 92），这5个氨基酸残基普遍存在于大肠杆菌的4种Na+转运载体蛋白、谷氨酸载体和脯氨酸载体中。大肠杆菌，以及兔和人肠道的Na+/葡萄糖共转运蛋白。我们建议，这个共识序列（或基序）可能发挥重要作用，在阳离子识别或结合的Na+/溶质同向转运反应。
The nucleotide sequence of the gltS gene coding for an Na+/glutamate symport carrier of Escherichia coli B has been determined, and the amino acid sequence of the carrier protein was deduced. The predicted glutamate carrier consists of 401 amino acids with a molecular weight of 42,455. A Shine-Dalgarno sequence and putative promoter sequences were found in the 5'-flanking region of the putative gltS gene. The predicted protein is very hydrophobic (73% nonpolar amino acids), and judging from its hydropathy profile, the protein is composed of 12 hydrophobic membrane-spanning segments with a mean length of 21.6 residues/segment. A typical rho-independent transcription termination signal was found downstream of the gltS gene. We found a conserved alignment of 5 amino acid residues (Gly42--Ala82-X-X-X-X-Leu87-X-X-X-Gly91-Arg92 ), which commonly exists in four Na+ symport carrier proteins, the glutamate carrier, and the proline carrier of E. coli, and the Na+/glucose co-transporters of rabbit and human intestines. We propose that this consensus sequence (or motif) may play an important role in cation recognition or binding in the Na+/solute symport reaction.