Nucleotide sequence analysis of seven Japanese isolates of Plantago asiatica mosaic virus (PlAMV): a unique potexvirus with significantly high genomic and biological variability within the species

Nucleotide sequence analysis of seven Japanese isolates of Plantago asiatica mosaic virus (PlAMV): a unique potexvirus with significantly high genomic and biological variability within the species
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DOI:
10.1007/s00705-007-1078-y
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发表时间:
2008-01-01
影响因子:
2.7
通讯作者:
Namba, S.
Namba, S.
中科院分区:
医学4区
文献类型:
--
作者:
Komatsu, K.;Yamaji, Y.;Namba, S.

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测定了6株日本百合分离株和1株报春花分离株车前草花叶病毒(PlAMV)的基因组序列。所有分离株的基因组大小为6102个核苷酸,包含Potexvirus属典型成员的5个开放阅读框。两两比较分析证实了PlAMV与郁金香病毒x的密切关系,但不同PlAMV分离株之间的同源性很低,包括外源分离株;俄罗斯分离株(PlAMV-Ru)、南迪纳分离株(PlAMV-Na)和日本分离株的RNA依赖性RNA聚合酶(RdRp)基因核苷酸序列同源性为75 ~ 77%。这些值在所有痘病毒的同一种不同分离株中是最低的。
The genomic sequences of Plantago asiatica mosaic virus (PlAMV), six lily isolates and one primrose isolate from Japan, were determined. The genomic size of all isolates was 6102 nucleotides, containing the five open reading frames typical of members of the genus Potexvirus. Pairwise comparison analyses confirmed the close relationship between PlAMV and tulip virus X. However, quite low identities were observed between different PlAMV isolates, including foreign isolates; nucleotide sequence identities of the RNA-dependent RNA polymerase (RdRp) gene between a Russian isolate (PlAMV-Ru), a Nandina isolate (PlAMV-Na) and Japanese isolates were 75-77%. These values were the lowest amongst different isolates of the same species of any potexviruses.