Preimplantation mouse embryo development as a target of the pesticide methoxychlor
Preimplantation mouse embryo development as a target of the pesticide methoxychlor
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DOI：
10.1016/s0890-6238(02)00095-3
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发表时间：
2003-01-01
影响因子：
3.3
通讯作者：
Eroschenko, VP
中科院分区：
文献类型：
--
作者：
Amstislavksy, SY;Kizilova, EA;Eroschenko, VP
本文研究了甲氧滴滴涕（MXC）和17 β-雌二醇（E）对小鼠附植前胚胎发育的影响。妊娠小鼠接受s.c.在妊娠第2-4天（plug =第1天）仅注射芝麻油、10 μ g E或0.5 mg纯化（95%）MXC。另一组仅在第2天接受2.5 μ g E的单剂量。基于妊娠雌性动物的平均体重，10 μ g雌二醇相当于0.33 mg/kg bw，2.5 μ g雌二醇相当于0.082 mg/kg bw，0.5 mg剂量的MXC相当于16.5 mg/kg bw。在第4天收集所有胚胎进行分析。MXC和两种雌二醇-17 β剂量均抑制胚胎发育为囊胚，减少胚胎细胞数量，并导致异常囊胚形成。高剂量的雌二醇-17 β显著增加了退化胚胎的百分比，并导致输卵管闭锁效应，胚胎滞留在输卵管中。与雌二醇-17 β相反，本研究中使用的剂量的MXC未改变胚胎的输卵管运输。与雌二醇-17 β相反，MXC增加了核碎裂和微核的百分比。在植入前胚胎中，MXC和雌二醇-17 β均抑制胚胎发育。然而，MXC的作用与雌二醇-17 β不同，表明作用机制不同，可能是由于细胞毒性和诱导细胞凋亡。(C)2002年爱思唯尔科技有限公司All rights reserved.
Effects of methoxychlor (MXC) and estradiol-17beta (E) were studied in mouse preimplantation embryos. Pregnant mice received s.c. injections of sesame oil only, 10 mug E, or 0.5 mg purified (95%) MXC on Days 2-4 of pregnancy (plug = Day 1). Another group received a single dose of 2.5 mug E on Day 2 only. Based on the average weight of pregnant females, 10 mug of estradiol was equivalent to 0.33 mg/kg of bw, 2.5 mug of estradiol was equivalent to 0.082 mg/kg of bw, and the 0.5-mg dose of MXC was equivalent to 16.5 mg/kg of bw. All embryos were collected for analyses on Day 4. MXC and both estradiol-17beta doses suppressed embryonic development to blastocyst, decreased embryo cell numbers, and caused abnormal blastocyst formation. The high estradiol-17beta dose significantly increased the percent degenerating embryos and caused a tube-locking effect, with retention of embryos in the oviduct. In contrast to estradiol-17beta, MXC at the dose used in this study did not alter tubal transport of embryos. Also in contrast to estradiol-17beta, MXC increased the percentage of nuclear fragmentation and micronuclei. In preimplantation embryos, MXC and estradiol-17beta both suppressed embryo development. MXC effects were, however, different from those of estradiol-17beta, indicating a difference in mechanism of action, possibly due to cytotoxicity and induction of apoptosis. (C) 2002 Elsevier Science Inc. All rights reserved.