Feruloylated Oligosaccharides Alleviate Dextran Sulfate Sodium-Induced Colitis in Vivo
Feruloylated Oligosaccharides Alleviate Dextran Sulfate Sodium-Induced Colitis in Vivo
复制标题
阿魏酰化低聚糖可减轻硫酸葡聚糖钠诱导的体内结肠炎
DOI：
10.1021/acs.jafc.9b03647
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发表时间：
2019
影响因子：
6.1
通讯作者：
Huang Junqing
中科院分区：
文献类型：
--
作者：
Xia Xichun;Zhu Leqing;Lei Zhiwei;Song Yueqi;Tang Fen;Yin Zhao;Wang Jing;Huang Junqing
炎症性肠病时，T淋巴细胞亚群失衡是导致肠道免疫功能紊乱和结肠组织损伤的重要原因。植物化学物质对细胞因子产生的调控为免疫调节剂的开发提供了广阔的前景。本文报道阿魏酸寡糖（FO）口服能有效减轻葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎。FOs降低了Th 17细胞的百分比，并下调了Th 17特异性细胞因子的产生。相比之下，FO增加了调节性T（Treg）细胞的百分比，并提高了DSS攻击小鼠结肠中Treg特异性细胞因子的产生。这些结果表明，FOs恢复了Th 17和Treg亚群的免疫平衡，从而改善了结肠炎的恶化。此外，FOs还能抑制树突状细胞分泌IL-23和IL-6，促进转化生长因子-β1（TGF-β1）的分泌，从而恢复Th 17和Treg细胞的免疫平衡。机制分析表明，FOs对IL-23和IL-6的调节与核因子-κ基因结合信号通路有关，对TGF-β1的调节与丝裂原活化蛋白激酶-激活蛋白1信号通路有关。综上所述，口服给药FO通过恢复Th 17和Treg细胞的免疫平衡对小鼠结肠炎发挥了有效的免疫调节作用。
The imbalance of T lymphocyte subsets substantially conduces to disturbed intestinal immune system and succeeding colonic tissue damage in inflammatory bowel diseases. It is considered that regulation of phytochemicals on cytokine production potentially provides a broad prospect for the exploitation of immunomodulatory agents. Here, we reported that oral administration of feruloylated oligosaccharides (FOs) effectively alleviated mice colitis disease induced by dextran sulfate sodium (DSS). FOs decreased the percentage of T helper (Th)17 cells and downregulated the production of Th17-specific cytokines. In contrast, FOs increased the percentage of regulatory T (Treg) cells and elevated the production of Treg-specific cytokines in colons of DSS-challenged mice. These results indicated that FOs restored the immunologic equilibrium of Th17 and Treg subsets, hereby ameliorating the deterioration of colitis. Furthermore, FOs diminished the secretion of interleukin (IL)-23 and IL-6 but enhanced the transforming growth factor-β1 (TGF-β1) in dendritic cells in vitro and in vivo, which contributed to the restoration of Th17 and Treg cells immune balance. The mechanistic analysis showed that the regulation of FOs on IL-23 and IL-6 was associated with the nuclear factor-κ-gene binding signaling pathway and TGF-β1 with mitogen-activated protein kinase-activator protein 1 signaling pathway. Taken together, oral administration of FOs exerted potent immunomodulatory effects against mice colitis via restoring the immune balance of Th17 and Treg cells.