Serological analysis of a cryptosporidiosis epidemic
Serological analysis of a cryptosporidiosis epidemic
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DOI：
10.1093/ije/29.2.376
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发表时间：
2000-04-01
影响因子：
7.7
通讯作者：
Calderon, RL
中科院分区：
文献类型：
--
作者：
Frost, FJ;Muller, T;Calderon, RL
背景1996年3月，安大略省Colling-wood的居民和游客中发生隐孢子虫病流行。在36例确诊病例中，55%是科林伍德的访客，57%的科林伍德居民病例年龄在10岁以下。科林伍德成年居民中报告的腹泻病发病率很低，这使政府官员和医生怀疑科林伍德是否发生了流行病。方法为更好地评价隐孢子虫的流行程度，采用Western blot法检测了89名柯林伍德成年居民和80名多伦多成年居民的隐孢子虫抗原组（15/17-kDa和27-kDa）的Ige抗体反应，分别在流行前、期间和之后进行常规检测。结果1996年1月1日至1996年6月17日采集的血清中，科林伍德市居民血清学应答率高于多伦多市（P < 0.002），平均血清学应答强度高于多伦多市（P < 0.001）。在首次病例报告后8周内采集的标本中，卷材林居民的血清学反应平均强度高于在此之前或之后采集的标本（15/ 17-MDa, P < 0.02; 27-kDa, P < 0.10）。结论这些升高的血清学反应表明，隐孢子虫感染在Collingwood居民中可能比疾病报告中更常见，与社区隐孢子虫病流行一致。在未来疑似隐孢子虫病的流行调查中应考虑类似的研究。
Background A cryptosporidiosis epidemic occurred among residents and visitors to Colling-wood, Ontario, during March 1996. Fifty-five per cent of 36 confirmed cases were Collingwood visitors and 57% of Collingwood resident cases were under 10 years of age. The low level of reported diarrhoeal illness among adult Collingwood residents caused government officials and physicians to question whether an epidemic had occurred in Collingwood.Methods To better evaluate the extent of the epidemic, anonymous surplus sera from 89 adult Collingwood residents, collected for routine tests prior to, during and after the epidemic, and from 80 adult Toronto residents were tested using a Western blot assay for Ige antibody response to two Cryptosporidium antigen groups (15/17-kDa and 27-kDa).Results For sera collected from 1 January 1996 to 17 June 1996, a higher fraction of Collingwood residents had a detectable serological response (P < 0.002) and the mean intensity of serological responses was higher for Collingwood than Toronto residents (P < 0.001). The mean intensity of serological responses for Coiling wood residents was higher in specimens drawn during the 8 weeks following the initial case reports compared to those drawn before or after this period (15/ 17-MDa, P < 0.02; 27-kDa, P < 0.10).Conclusions These elevated serological responses indicate that Cryptosporidium infections among Collingwood residents likely occurred more commonly than illness reports suggested, consistent with a community-wide cryptosporidiosis epidemic. Similar studies should be considered in future suspected cryptosporidiosis epidemic investigations.