Nanosilver-stimulated heterophil extracellular traps promoted liver and kidney injury in chicken.
Nanosilver-stimulated heterophil extracellular traps promoted liver and kidney injury in chicken.
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DOI：
10.1016/j.jinorgbio.2022.111838
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发表时间：
2022-04
影响因子：
3.9
通讯作者：
W. Liu;Wenlong Huang;Shurou Li;Haiguang Zhao;Liqiang Jiang;Jingnan Xu;Xinxin Gao;Zhengtao Yang;Zhengkai Wei
中科院分区：
文献类型：
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作者：
W. Liu;Wenlong Huang;Shurou Li;Haiguang Zhao;Liqiang Jiang;Jingnan Xu;Xinxin Gao;Zhengtao Yang;Zhengkai Wei
纳米银是直径<100 nm的金属银单体，具有优异的抗菌活性，广泛应用于医药卫生、日常生活饮用水消毒和畜禽养殖饲料添加剂等领域。嗜异性细胞外陷阱是鸡天然免疫的重要组成部分，具有良好的抗菌作用，但其过度释放会造成组织损伤。纳米银过量对鸡产生毒性作用，而纳米银对鸡的免疫毒性作用尚未报道。本研究探讨了纳米银诱导的组胺对鸡肝、肾损伤的影响，并进一步探讨了纳米银诱导组胺释放的分子机制。结果表明，纳米银可显著上调血清肝素水平，并引起肝、肾损害。纳米银诱导的HSP 70的经典结构也被观察到，纳米银诱导的HSP 70依赖于活性氧（ROS）、细胞外调节蛋白激酶（ERK）1/2、p38和糖酵解途径。综上所述，本研究表明纳米银诱导了Hisp的释放，但过量的Hisp释放也会对鸡造成伤害。这也有助于理解适度应用纳米银的重要性，这可以提高动物免疫力，但避免负面影响，以保障家禽养殖的经济效益。
Nanosilver is a metallic silver monomer with a diameter of <100 nm, which has excellent antibacterial activity and is widely used in the fields of medicine and sanitation, disinfection of drinking water in daily life and feed additives in livestock and poultry farming. Heterophil extracellular traps (HETs) are an important part of innate immunity in chickens and have an excellent antimicrobial effect, but their excessive release caused tissue damage. Nanosilver overdose caused toxic effects in chickens, while immunotoxic effects of nanosilver on chickens have not been reported. In this study, we explored the effects of nanosilver-induced HETs on chicken liver and kidney damage and further investigated the molecular mechanism of nanosilver-induced HETs release. The results showed that nanosilver significantly upregulated serum HETs and caused liver and kidney damage. The classical structure of nanosilver-induced HETs was also observed, and nanosilver-induced HETs were dependent on reactive oxygen species (ROS), extracellular regulatory protein kinase (ERK)1/2, p38 and glycolysis pathways. In summary, this research suggests that nanosilver induced HETs release, but excessive HETs release also caused damage to chicken. It also helps to understand the importance of moderate application of nanosilver, which may improve animal immunity but avoid negative effects in safeguarding the economic efficiency of poultry farming.