A family of DNA repeats in Aspergillus nidulans has assimilated degenerated retrotransposons
A family of DNA repeats in Aspergillus nidulans has assimilated degenerated retrotransposons
复制标题
DOI：
10.1007/s004380100484
复制
发表时间：
2001-07-01
影响因子：
3.1
通讯作者：
Aleksenko, A
中科院分区：
文献类型：
--
作者：
Nielsen, ML;Hermansen, TD;Aleksenko, A
在向构巢曲霉的染色体IV的着丝粒进行染色体游走的过程中，分离出几个交叉杂交的基因组粘粒克隆。两个这样的克隆的限制性酶切图谱显示，它们的限制性酶切图谱在至少15 kb的区域内相似，表明存在大的重复序列。通过测序和Southern分析进一步研究重复的性质。该研究揭示了一个具有高度序列相似性的长分散重复序列家族。重复序列的数量和位置在野生分离株之间不同。两个拷贝的重复序列含有简并的长末端重复序列（LTR）反转录转座子，它们被命名为Dane1和Dane2（对于（D）在bar下的regenerated（A）在bar下的spereneous（n）在bar下的idulans（e）在bar下的lement）。退化的模式表明，一个过程类似于重复诱导的点突变（RIP）现象，首先在粗糙脉孢菌中描述，可能已经在A。nidulans。这些数据表明，这个家庭的重复已经同化的移动的元素，随后退化，但随后经历了进一步的复制作为主机重复的一部分。
In the course of a chromosomal walk towards the centromere of chromosome IV of Aspergillus nidulans, several cross-hybridizing genomic cosmid clones were isolated. Restriction mapping of two such clones revealed that their restriction patterns were similar in a region of at least 15 kb, indicating the presence of a large repeat. The nature of the repeat was further investigated by sequencing and Southern analysis. The study revealed a family of long dispersed repeats with a high degree of sequence similarity. The number and location of the repeats vary between wild isolates. Two copies of the repeat contained degenerated long-terminal-repeat (LTR) retrotransposons, which were named Dane1 and Dane2 (for (D) under bar egenerated (A) under bar spergillus (n) under bar idulans (e) under bar lement). The pattern of degeneration suggested That a process similar to the repeat-induced point-mutation (RIP) phenomenon, first described in Neurospora crassa, may have operated in A. nidulans. The data indicate that this family of repeats has assimilated mobile elements that subsequently degenerated but then underwent further duplications as a part of the host repeats.