Evidence that rseC, a gene in the rpoE cluster, has a role in thiamine synthesis in Salmonella typhimurium
Evidence that rseC, a gene in the rpoE cluster, has a role in thiamine synthesis in Salmonella typhimurium
复制标题
DOI：
10.1128/jb.179.21.6862-6864.1997
复制
发表时间：
1997-10-01
影响因子：
3.2
通讯作者：
Downs, DM
中科院分区：
文献类型：
--
作者：
Beck, BJ;Connolly, LE;Downs, DM
在鼠伤寒沙门氏菌中，氨基咪唑核苷酸（AIR）转化为硫胺素的4-氨基-5-羟甲基-2-甲基嘧啶（HMP）部分所需的遗传基因座和生化反应仍然未知。初步遗传分析表明，可能有一种以上的途径负责从ATR合成HMP，并且这些途径的功能取决于UR的可用性，UR是通过嘌呤途径或通过purF非依赖性替代嘧啶生物合成（APE）途径合成的（L.，Petersen和D.唐斯，J.，Bacteriol. 178：5676-5682，1996）。rseB中的插入，在57分钟时rpoE rseABC基因簇中的第三个基因，阻止了purl：突变体中HMP的合成。互补分析表明，purF rseB菌株的HMP需要是由于下游rseC基因上的rseB中的插入的极性。RseC在硫胺素合成中的作用与rpoE无关。
In Salmonella typhimurium, the genetic loci and biochemical reactions necessary for the conversion of aminoimidazole ribotide (AIR) to the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine remain unknown. Preliminary genetic analysis indicates that there may be more than one pathway responsible for the synthesis of HMP from ATR and that the function of these pathways depends on the availability of UR, synthesized by the purine pathway or by the purF-independent alternative pyrimidine biosynthetic (APE) pathway (L., Petersen and D. Downs, J., Bacteriol. 178:5676-5682, 1996). An insertion in rseB, the third gene in the rpoE rseABC gene cluster at 57 min, prevented HMP synthesis in a purl: mutant, Complementation analysis demonstrated that the HMP requirement of the purF rseB strain was due to polarity of the insertion in rseB on the downstream rseC gene. The role of RseC in thiamine synthesis was independent of rpoE.