Serologic association between human papillomavirus type 16 infection and esophageal cancer in Shaanxi Province, China
Serologic association between human papillomavirus type 16 infection and esophageal cancer in Shaanxi Province, China
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DOI：
10.1093/jnci/88.20.1467
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发表时间：
1996-10-16
影响因子：
10.3
通讯作者：
Schiller, JT
中科院分区：
文献类型：
--
作者：
Han, CL;Qiao, GB;Schiller, JT
背景资料：一些国家（如中国）食管癌患病率存在较大的地理差异，表明环境风险因素可能在该疾病的发生中起重要作用，一些研究表明，人乳头瘤病毒（HPV）的生殖器粘膜株与该癌症的病因有关，目的：我们在中国陕西省食管癌高危人群中进行了一项病例对照研究，以评估该疾病与HPV 16型（HPV 16）感染的相关性，最常见的癌症相关的生殖器粘膜HPV类型，方法：从陕西省西安市两家医院的食管癌患者中筛选出90例食管癌患者和121例无癌对照者，对照组与病例组按年龄和性别配对，所有研究对象均抽取血液标本，通过使用杆状病毒衍生的HPV 16病毒样颗粒作为抗原的酶联免疫吸附测定（ELISA）来确定血清样品中HPV 16抗体的存在，使用牛乳头瘤病毒1型（BPV 1）病毒样颗粒作为抗原的类似ELISA控制HPV 16血清反应性的特异性，将来自HPV 16和BPV 1测定的数据相对于在每个测定中使用已知HPV 16血清反应性的对照血清获得的结果进行标准化，计算比值比（OR）和95%置信区间（CI）以检查HPV 16血清反应性与食管癌之间的关联，报告的P值是双侧的。结果如下：癌症患者对HPV 16病毒样颗粒的平均血清反应性显著高于对照组（平均值+/-标准差= 0.85 +/- 0.22对0.74 +/- 0.18; P
Background: The existence of large geographic variations in the prevalence of esophageal cancer in some countries, such as China, indicates that environmental risk factors may be important in the development of this disease, Some studies have implicated genital-mucosal strains of human papillomaviruses (HPVs) in the etiology of this cancer, Purpose: We conducted a case-control study in Shaanxi Province, China, an area with a population at high risk for esophageal cancer, to assess the association of this disease with infection by HPV type 16 (HPV16), the most common cancer-associated genital-mucosal HPV type, Methods: Ninety individuals with esophageal cancer and 121 cancer-free control subjects were identified among the patients in two hospitals in Xi'an, Shaanxi Province, The control subjects were matched to the case patients on the basis of age and sex, Blood specimens were drawn from all study subjects, and serum was isolated by routine methods, The presence of HPV16 antibodies in serum samples was determined by use of an enzyme-linked immunosorbent assay (ELISA) that used baculovirus-derived HPV16 virus-like particles as the antigen, A similar ELISA that used bovine papillomavirus type 1 (BPV1) virus-like particles as the antigen controlled for the specificity of HPV16 seroreactivity, Data from the HPV16 and the BPV1 assays were normalized with respect to results obtained in each assay with a control serum of known HPV16 seroreactivity, Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to examine the association between HPV16 seroreactivity and esophageal cancer, Reported P values are two-sided. Results: The mean seroreactivity to HPV16 virus-like particles was significantly higher for the cancer patients than for the control subjects (mean value +/- standard deviation = 0.85 +/- 0.22 versus 0.74 +/- 0.18; P