Effect of exercise from a very early stage after intracerebral hemorrhage on microglial and macrophage reactivity states in rats
Effect of exercise from a very early stage after intracerebral hemorrhage on microglial and macrophage reactivity states in rats
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DOI：
10.1097/wnr.0000000000001782
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发表时间：
2022-04
期刊：
影响因子：
1.7
通讯作者：
K. Tamakoshi;Madoka Maeda;Nae Murohashi;Ami Saito
中科院分区：
文献类型：
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作者：
K. Tamakoshi;Madoka Maeda;Nae Murohashi;Ami Saito
目的探讨脑出血(ICH)后早期运动对大鼠小胶质细胞和巨噬细胞的影响。左侧纹状体内注射胶原酶溶液诱导脑出血。方法大鼠随机分为对照组(假手术组)、脑出血非运动组(ICH组)和脑出血早期运动组(ICH+VET组)。脑出血+VET组于脑出血后6h、2 4h、2~6d进行平板运动。分别于脑出血后3h、25h、7d采用梯形试验和旋转棒试验进行运动功能评定。于术后第8天采集运动后脑组织，观察损伤体积。很早的锻炼会暂时加重运动功能障碍。分别于脑出血后6h、24h和8d检测巨噬细胞和小胶质细胞标志物CD80、CD163和TMEM119的蛋白表达水平。脑出血后第8天进行NeuN、GFAP和PSD95蛋白分析。结果脑出血后第8天，脑出血组和脑出血+VET组的病灶体积差异无统计学意义。从很早的阶段开始运动就能阻止CD163蛋白表达的升高。结论早期运动可抑制抗炎相关巨噬细胞/小胶质细胞的激活。
Objective This study investigated the effects of exercise, starting very early after intracerebral hemorrhage (ICH), on microglia and macrophages in a rat model. Collagenase solution was injected into the left striatum to induce ICH. Methods Rats were randomly assigned to receive placebo surgery without exercise (sham surgery), ICH without exercise (ICH), or ICH with very early exercise (ICH + VET). The ICH + VET group was subjected to treadmill running 6 h, 24 h, and days 2–6 after ICH. Motor function assessment was performed using the ladder test and rotarod test 3 h, 25 h, and 7 days after ICH. Postexercise brain tissue was collected on day 8 after surgery to investigate the lesion volume. Very early exercise temporarily worsened motor dysfunction. The protein expression levels of the macrophage and microglial markers CD80, CD163, and TMEM119 were analyzed 6 h, 24 h, and 8 days after ICH. Protein analysis of NeuN, GFAP, and PSD95 was also performed on day 8 after ICH. Results There was no significant difference in lesion volume between the ICH and ICH + VET groups on day 8 after ICH. Exercise from very early stage prevented elevated CD163 protein expression. Conclusion Very early exercise may inhibit the activation of anti-inflammatory-associated macrophages/microglia.