Ultrastructural studies of gametogenesis in Laminaria angustata (Laminariales, Phaeophyta) regulated by iron concentration in the medium

Ultrastructural studies of gametogenesis in Laminaria angustata (Laminariales, Phaeophyta) regulated by iron concentration in the medium
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培养基中铁浓度调节海带(Laminariales,Phaeophyta)配子发生的超微结构研究

DOI:
10.2216/i0031-8884-23-3-331.1
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发表时间:
1984
期刊:
影响因子:
1.6
通讯作者:
Y. Sakai
Y. Sakai
中科院分区:
生物学3区
文献类型:
--
作者:
T. Motomura;Y. Sakai

文献摘要

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摘要将雌、雄配子体转移到含有2 mg L、−、1-Fe的改良ASP12NTA培养基中,诱导雌、雄配子体发生。超微结构变化描述了生殖成熟的四个连续阶段的每一个阶段。在营养配子体中,高尔基体摘除特有的小电子致密小泡,异染色质出现在细胞核的几个位置。在卵子发育早期,高尔基体数量增多,活跃地产生大的电子透明小泡,核染色质弥散。在成熟的卵原细胞中,内质网发达,稍有扩张,并出现大量嗜欧性物质。在被解放的卵子中,这些嗜奥斯性包裹体逐渐减少。在精子发育的早期,高尔基体产生大的小泡,但不会像卵子形成那样在数量上增加。核分裂后,每个雄蕊中形成一个精子,细胞核中的染色质通常是...
Abstract Gametogenesis is induced in female and male gametophytes of L. angustata Kjellman by transferring them to modified ASP-12 NTA medium containing 2 mg l−1 Fe. Ultrastructural changes are described for each of the four successive stages of reproductive maturation. In vegetative gametophytes, the Golgi bodies pinch off characteristic small electron dense vesicles, and heterochromatin occurs at several sites in the nuclei. In early egg development, the Golgi bodies increase in number and actively produce large electron-transparent vesicles and nuclear chromatin disperses. In the mature oogonium, the endoplasmic reticulum becomes well-developed and expands slightly, and a large amount of osmiophilic material occurs. In the liberated egg, these osmiophilic inclusions decrease grapually. In early sperm development, Golgi bodies produce large vesicles but do not increase in number as in egg formation. After nuclear divisions, a sperm is formed in each antheridium and chromatin in the nucleus is generally ...