Genetic and behavioral differences among five inbred mouse strains commonly used in the production of transgenic and knockout mice
Genetic and behavioral differences among five inbred mouse strains commonly used in the production of transgenic and knockout mice
复制标题
DOI：
10.1111/j.1601-183x.2004.00064.x
复制
发表时间：
2004-06-01
影响因子：
2.5
通讯作者：
Geistfeld, JG
中科院分区：
文献类型：
--
作者：
Bothe, GWM;Bolivar, VJ;Geistfeld, JG
五种常用的转基因和基因敲除生产的小鼠品系进行了比较，遗传背景和行为。这些菌株是：C57 BL/6 J、C57 BL/6 NTac、129 P3/J（以前为129/J）、129 S6/SvEvTac（以前为129/SvEvTac）和FVB/NTac。342个微卫星标记的基因型和性能在三个行为测试（转棒，旷场活动和习惯化，上下文和线索恐惧条件反射）进行了测定。C57 BL/6 J和C57 BL/6 NTac是真正的亚株，它们之间只有12个微卫星差异。鉴于遗传背景的数据，可以预测两个C57 BL/6亚株在行为上应该非常相似。事实上，C57 BL/6 J和C57 BL/6 NTac之间没有显著的行为差异。与其他129株菌株的文献报道相反，129 S6/ SvEvTac通常与C57 BL/6菌株相似，只是活性较低。FVB/NTac显示旋转杆学习受损和线索恐惧条件反射。因此，当研究人员想要在这三种行为测试中的任何一种中评估他们的小鼠时，推荐129 S6/SvEvTac和C57 BL/6作为靶向突变的背景品系。然而，FVB/NTac背景下的任何转基因都应转移至B6。利用总距离、中心距、速度和垂直活动等参数分析了对开阔场地的适应性。与早期的研究相反，我们发现，所有菌株习惯于开放领域的至少两个这些参数（中心距离和速度）。
Five strains of mice commonly used in transgenic and knockout production were compared with regard to genetic background and behavior. These strains were: C57BL/6J, C57BL/6NTac, 129P3/J (formerly 129/J), 129S6/SvEvTac (formerly 129/SvEvTac) and FVB/NTac. Genotypes for 342 microsatellite markers and performance in three behavioral tests (rotorod, open field activity and habituation, and contextual and cued fear conditioning) were determined. C57BL/6J and C57BL/ 6NTac were found to be true substrains; there were only 12 microsatellite differences between them. Given the data on the genetic background, one might predict that the two C57BL/6 substrains should be very similar behaviorally. Indeed, there were no significant behavioral differences between C57BL/6J and C57BL/6NTac. Contrary to literature reports on other 129 strains, 129S6/ SvEvTac often performed similarly to C57BL/6 strains, except that it was less active. FVB/NTac showed impaired rotorod learning and cued fear conditioning. Therefore, both 129S6/SvEvTac and C57BL/6 are recommended as background strains for targeted mutations when researchers want to evaluate their mice in any of these three behavior tests. However, any transgene on the FVB/NTac background should be transferred to B6. Habituation to the open field was analyzed using the parameters: total distance, center distance, velocity and vertical activity. Contrary to earlier studies, we found that all strains habituated to the open field in at least two of these parameters (center distance and velocity).