Intracellular metabolite profiling of Saccharomyces cerevisiae evolved under furfural.
Intracellular metabolite profiling of Saccharomyces cerevisiae evolved under furfural.
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DOI：
10.1111/1751-7915.12465
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发表时间：
2017-03
影响因子：
5.7
通讯作者：
Kim KH
中科院分区：
文献类型：
--
作者：
Jung YH;Kim S;Yang J;Seo JH;Kim KH
糠醛是预处理水解产物中最常见的抑制剂之一，可减少酵母的细胞生长和乙醇产量。进化工程已被用作获得具有糠醛耐受性的酵母菌株的选择方案。然而，酿酒酵母在进化过程中代谢物水平对糠醛的反应仍不清楚。在这项研究中，应用进化工程和代谢组学分析来确定糠醛对酵母的影响及其对持续接触糠醛的代谢反应。在糠醛存在下进行 50 次连续培养转移后，进化的菌株获得了在糠醛胁迫下稳定管理其生理状态的能力。总共鉴定出 98 种代谢物，其丰度分布表明酵母代谢受到全球调控。在糠醛应激下，应激保护分子和辅因子相关机制主要在亲本菌株中诱导产生。然而，在糠醛胁迫下的进化过程中，酿酒酵母经历了全局代谢分配以快速克服压力，特别是通过维持与能量产生、辅因子再生和细胞损伤恢复相关的较高水平的代谢物。在本研究中，绘制进化工程赋予的糠醛耐受机制将有助于合理设计代谢工程酵母。
Furfural, one of the most common inhibitors in pre‐treatment hydrolysates, reduces the cell growth and ethanol production of yeast. Evolutionary engineering has been used as a selection scheme to obtain yeast strains that exhibit furfural tolerance. However, the response of Saccharomyces cerevisiae to furfural at the metabolite level during evolution remains unknown. In this study, evolutionary engineering and metabolomic analyses were applied to determine the effects of furfural on yeasts and their metabolic response to continuous exposure to furfural. After 50 serial transfers of cultures in the presence of furfural, the evolved strains acquired the ability to stably manage its physiological status under the furfural stress. A total of 98 metabolites were identified, and their abundance profiles implied that yeast metabolism was globally regulated. Under the furfural stress, stress‐protective molecules and cofactor‐related mechanisms were mainly induced in the parental strain. However, during evolution under the furfural stress, S. cerevisiae underwent global metabolic allocations to quickly overcome the stress, particularly by maintaining higher levels of metabolites related to energy generation, cofactor regeneration and recovery from cellular damage. Mapping the mechanisms of furfural tolerance conferred by evolutionary engineering in the present study will be led to rational design of metabolically engineered yeasts.