Cd2+ -promoted mitochondrial permeability transition: a comparison with other heavy metals.
Cd2+ -promoted mitochondrial permeability transition: a comparison with other heavy metals.
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DOI：
10.18388/abp.2004_3590
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发表时间：
2004
影响因子：
1.7
通讯作者：
E. Belyaeva;V. Glazunov;S. Korotkov
中科院分区：
文献类型：
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作者：
E. Belyaeva;V. Glazunov;S. Korotkov
我们比较了Cd（2+）、Hg（2+）和Cu（2+）在不加Ca（2+）和P（i）的情况下对离体大鼠肝线粒体的作用。重金属离子浓度依赖性地产生：（1）增强膜透性，表现为线粒体肿胀和基础呼吸激活，（2）抑制解偶联剂刺激的呼吸，（3）膜电位耗散。在NH（4）NO（3）介质中，Cu（2+）对基础呼吸的刺激作用最大，对DNP解偶联呼吸的抑制作用最小; Cd（2+）对解偶联呼吸的抑制作用最强，对呼吸的溶胀作用最大。在高浓度[Cd（2+）]和[Hg（2+）]后加入二硫苏糖醇可诱导基础呼吸释放，且对CsA不敏感。
We compared action of Cd(2+), Hg(2+), and Cu(2+) on isolated rat liver mitochondria in the absence of added Ca(2+) and P(i). The heavy-metal ions produced dose-dependently: (1) enhanced membrane permeabilization manifested in mitochondrial swelling and activation of basal respiration, (2) inhibition of uncoupler-stimulated respiration, and (3) membrane potential dissipation. Among the metals, Cu(2+) exhibited maximal stimulatory effect on basal respiration and minimal inhibitory action on DNP-uncoupled respiration whilst Cd(2+) promoted the strongest depression of uncoupled respiration and the largest swelling in NH(4)NO(3) medium. Dithiothreitol induced a basal respiration release if added after high [Cd(2+)] and [Hg(2+)], and the stimulation was CsA-insensitive.