Dehydration and Estrous Staging in the Rat Larynx: an in vivo Prospective Investigation
Dehydration and Estrous Staging in the Rat Larynx: an in vivo Prospective Investigation
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DOI：
10.1016/j.jvoice.2019.06.009
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发表时间：
2021-01-13
期刊：
影响因子：
2.2
通讯作者：
Sivasankar, M. Preeti
中科院分区：
文献类型：
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作者：
Cox, Abigail;do Nascimento, Naila Cannes;Sivasankar, M. Preeti
Objective.这项新的研究试图解开声带中水合状态和发情周期之间的关联，因为据报道，在发情周期和脱水期间，声音会在扬声器中发生负面变化。我们假设声带组织的形态会根据水合状态而改变，并且这些变化会随着发情周期而变化。前瞻性体内研究设计。本研究使用雌性Sprague道利大鼠（n = 30）。16只大鼠全身脱水，通过禁水使体重平均减轻10%（体重减轻范围：8%-13%）。将14只大鼠分配至水分充足的对照条件。通过阴道涂片的细胞学评价确定雌性Sprague道利大鼠（n = 30）的发情期。安乐死后，准备喉，用苏木精和伊红、Masson三色和阿辛蓝（pH 2.5）进行组织学染色。为了定量透明质酸，在甲状腺素酸酶孵育前和孵育后完成阿辛蓝染色。用图像分析算法定量染色百分比的变化，并报告为透明质酸量。相对胶原分布（脱水指数）、透明质酸量和组织形态是结果指标。全身性脱水与大鼠声带固有层中透明质酸量的变化相关。脱水并没有显着影响胶原蛋白的分布，也没有组织形态。单独的动情期不影响声带透明质酸的数量，不改变组织形态，也不改变胶原的分布。大鼠声带固有层中透明质酸量的减少可能在全身性脱水期间的组织液平衡中发挥作用。未来的研究将扩大这项工作，调查声带细胞外基质的其他成分，以充分阐明水化状态对声带的影响。
Objective. This novel study sought to untangle the association between hydration state and the estrous cycle in the vocal folds, since the voice is reported to negatively change in speakers during the estrous cycle and with dehydration. We hypothesized that there would be alterations in vocal fold tissue morphology depending on hydration state and that these changes would vary with the estrous cycle.Study Design. Prospective, in vivo study design.Methods. Female Sprague Dawley rats (n = 30) were used in this study. Sixteen rats were systemically dehydrated to an average of 10% reduction in body weight by withholding water (range of body weight loss: 8%-13%). Fourteen rats were assigned to euhydrated, control condition. Estrous stage of female Sprague Dawley rats (n = 30) was determined via cytological evaluation of vaginal smears. Following euthanization, larynges were prepared for histological staining with hematoxylin and eosin, Masson's trichrome and alcian blue (pH 2.5). To quantify hyaluronan, alcian blue staining was completed pre- and posthyaluronidase incubation. The change in staining percent was quantified with image analysis algorithms and reported as the hyaluronan quantity. Relative collagen distribution (index of dehydration), hyaluronan quantity, and tissue morphology were the outcome measures.Results. Systemic dehydration was associated with changes in hyaluronan quantity in the rat vocal fold lamina propria. Dehydration did not significantly affect the collagen distribution nor the tissue morphology. Estrous stage alone does not impact the quantity of vocal fold hyaluronan, alter tissue morphology, or change collagen distribution.Conclusion. Decreases in hyaluronan quantity in the lamina propria of the rat vocal fold may play a role in tissue fluid balance during systemic dehydration. Future studies will expand this work to investigate additional components of the vocal fold extracellular matrix to fully elucidate the impact of hydration state on the vocal fold.