A proposed model for the regulation of phosphofructokinase and fructose 1,6‐bisphosphatase based on their reciprocal anomeric specificities
A proposed model for the regulation of phosphofructokinase and fructose 1,6‐bisphosphatase based on their reciprocal anomeric specificities
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基于磷酸果糖激酶和果糖 1,6-双磷酸酶相互异头特异性的拟议调节模型
DOI：
10.1016/0014-5793(77)80689-3
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发表时间：
1977
期刊：
影响因子：
3.5
通讯作者：
E. S. Younathan
中科院分区：
文献类型：
--
作者：
T. Koerner;R. Voll;E. S. Younathan
碳水化合物代谢的一个经常被忽视的方面是，溶液中的大多数碳水化合物是几种结构和构型异构体（无环形式和异头物）的混合物，每种异构体以不同的浓度存在，并且能够对酶催化和异构位点表现出不同的亲和力和反应性（异头特异性）。在本文中，以磷酸果糖激酶/果糖 1, 6-二磷酸酶对为例，认为碳水化合物的这种特征异构性可能为代谢它们的酶的调节提供基础。几位研究人员指出 anomedc 特异性可能发挥调节作用的可能性 [1-5]；然而，到目前为止，还没有提出任何机制来表明如何实现这一监管。本通讯中提出的调节机制的基础是最近收获的有关溶液中碳水化合物的异头平衡的数据（在[6, 7]中综述）和葡萄糖代谢酶的异头特异性（在[2, 4]中综述）。 13C NMR 光谱表明 D-果糖 6-磷酸 (F6P) 和 D-果糖 1, 6-二磷酸 (FBP) 是水溶液中的平衡混合物（图 l），其组成为
An often overlooked aspect of carbohydrate metabolism is that most carbohydrates in solution are mixtures of several constitutional and configurational isomers (acyclic forms and anomers), each present in different concentrations and capable of displaying a different affinity and reactivity for enzyme catalytic and allostenc sites (anomeric specificity). In this paper, the possibility is considered that this characteristic isomerism of carbohydrates might provide the basis for regulation of the enzymes that metabolize them, using the example of the phosphofructokinase/fructose 1, 6-bisphosphatase enzyme pair. Several investigators have pointed out the possibility that anomedc specificity may play a regulatory role [1-5]; however, until now no mechanism has been proposed to show how this regulation may be achieved. The basis for the regulatory mechanism proposed in this communication is the recent harvest of data concerning the anomeric equilibria of carbohydrates in solution (reviewed in [6, 7]) and the anomeric specificity of the enzymes of glucose metabolism (reviewed in [2, 4]). D-Fructose 6-phosphate (F6P) and D-fructose 1, 6-bisphosphate (FBP) have been shown by laC NMR spectroscopy to be equilibrated mixtures in aqueous solution (fig. l), composed of