TRANSFECTION AND TRANSFORMATION OF AGROBACTERIUM-TUMEFACIENS
TRANSFECTION AND TRANSFORMATION OF AGROBACTERIUM-TUMEFACIENS
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DOI：
10.1007/bf00267408
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发表时间：
1978-01-01
期刊：
影响因子：
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通讯作者：
SCHELL, J
中科院分区：
文献类型：
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作者：
HOLSTERS, M;DEWAELE, D;SCHELL, J
采用Dityatkin等人（1972）的冻融转染方法对a进行转染转化。农。用温带噬菌体PS8cc186的DNA转染菌株B6S3和B6-6的频率最高为2 10-7个/总受体群体。用P型质粒RP4转染菌株GV3100时，每总受体群体最多可获得3.5 10-7个转化子。在菌株GV3100中引入农杆菌ti质粒，最高效率为4.5 10-8。这些实验提供了进一步的证据，证明ti质粒是导致a肿瘤因子致癌特性的原因，以及它在冠瘿植物细胞中诱导“opine”合成的能力。
The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation ofA. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10-7transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10-7transformants per total recipient population was obtained.AgrobacteriumTi-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10-8. These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties ofA tumefaciensand for its capacity to induce “opine” synthesis in Crown-gall plant cells.