Promoting neuroregeneration by applying dynamic magnetic fields to a novel nanomedicine: Superparamagnetic iron oxide (SPIO)-gold nanoparticles bounded with nerve growth factor (NGF).
Promoting neuroregeneration by applying dynamic magnetic fields to a novel nanomedicine: Superparamagnetic iron oxide (SPIO)-gold nanoparticles bounded with nerve growth factor (NGF).
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DOI：
10.1016/j.nano.2018.03.004
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
Muzhaozi Yuan;Ya Wang;Yi-Xian Qin
中科院分区：
文献类型：
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作者：
Muzhaozi Yuan;Ya Wang;Yi-Xian Qin
神经再生在神经科学中对治疗神经退行性疾病提出了重大挑战。本研究的目的是评估神经生长因子（NGF）功能化的超顺磁性氧化铁（SPIO）-金（Au）纳米药物在外磁场（MF）下可以刺激神经元生长和分化的假设，动态MF优于静态MF。SPIO-Au核壳纳米粒子（直径：20.8nm）具有诸如均匀的准球形形状、窄的尺寸分布、优异的稳定性和低毒性（5天存活率>96%）的优点。NGF功能化增强了细胞摄取。在PC-12细胞上的实验和在细胞骨架力模型上的理论揭示了使用NGF功能化的SPIO-Au NPs由静态和动态MFs驱动的神经元生长和取向的促进。更重要的是，通过旋转的动态MF比静态MF表现得更好，即，细胞分化率提高58%，神经突起长度延长63%。
Neuroregeneration imposes a significant challenge in neuroscience for treating neurodegenerative diseases. The objective of this study is to evaluate the hypothesis that the nerve growth factor (NGF) functionalized superparamagnetic iron oxide (SPIO)-gold (Au) nanomedicine can stimulate the neuron growth and differentiation under external magnetic fields (MFs), and dynamic MFs outperform their static counterparts. The SPIO-Au core-shell nanoparticles (NPs) (Diameter: 20.8 nm) possessed advantages such as uniform quasi-spherical shapes, narrow size distribution, excellent stabilities, and low toxicity (viability >96% for 5 days). NGF functionalization has enhanced the cellular uptake. The promotion of neuronal growth and orientation using NGF functionalized SPIO-Au NPs, driven by both the static and dynamic MFs, was revealed experimentally on PC-12 cells and theoretically on a cytoskeletal force model. More importantly, dynamic MFs via rotation performed better than the static ones, i.e., the cellular differentiation ratio increased 58%; the neurite length elongation increased 63%.