Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth
Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth
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DOI：
10.1128/jb.01736-06
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发表时间：
2007-05-01
影响因子：
3.2
通讯作者：
Brul, Stanley
中科院分区：
文献类型：
--
作者：
Keijser, Bart J. F.;Ter Beek, Alex;Brul, Stanley
枯草芽孢杆菌在营养耗尽时形成休眠孢子。在有利的环境条件下，孢子打破休眠，恢复生长，这一过程称为孢子萌发和生长。为了阐明休眠孢子向活跃生长的营养细胞转变过程中发生的生理过程，我们结合显微镜、胞外代谢物分析和全基因组转录分析，以时间依赖的方式研究了这一过程。结果表明，在休眠孢子中存在大量的晚期产孢子转录本。此外，研究结果表明，枯草杆菌存在一个复杂的、调控良好的孢子生长计划，涉及至少30%的枯草杆菌基因组的时间表达。
Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we studied this process in a time-dependent manner by a combination of microscopy, analysis of extracellular metabolites, and a genome-wide analysis of transcription. The results indicate the presence of abundant levels of late sporulation transcripts in dormant spores. In addition, the results suggest the existence of a complex and well-regulated spore outgrowth program, involving the temporal expression of at least 30% of the B. subtilis genome.