COLONIZATION OF MICE BY CAMPYLOBACTER-JEJUNI
COLONIZATION OF MICE BY CAMPYLOBACTER-JEJUNI
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DOI：
10.1128/iai.57.8.2279-2282.1989
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发表时间：
1989-08-01
影响因子：
3.1
通讯作者：
PONGPECH, P
中科院分区：
文献类型：
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作者：
JESUDASON, MV;HENTGES, DJ;PONGPECH, P
链霉素处理和未处理的瑞士白色小鼠在用1至1011个空肠弯曲杆菌两种菌株中的任一种的活菌进行口胃攻击时均不规则地定殖。当攻毒剂量为1010或更高时，偶尔从这些动物的肠道部分回收到数量范围为101 - 103/g的微生物。当无菌小鼠用108个菌株的微生物进行攻击时，所有动物的整个肠道都被C.空肠数量为104 ~ 109个/g。盲肠是最严重的殖民地。两株C.空肠在布鲁氏杆菌肉汤中厌氧繁殖，除了当肉汤在pH 6.75时含有60.80 μ eq/ml的总挥发性脂肪酸（VFA）时，模拟未处理小鼠盲肠中的条件，或者当肉汤在pH 7.04时含有21.63 μ eq/ml时，模拟链霉素处理小鼠盲肠中的条件。然而，在pH 7.02的不含VFA的布鲁氏菌肉汤中进行微氧孵育，模拟无菌小鼠盲肠中的条件，发生了活跃的增殖。结果表明，在厌氧条件下操作的VFA存在于链霉素处理和未处理的常规小鼠的肠道中，干扰C.空肠。另一方面，在无菌小鼠肠道的较高氧化还原电位下，在不存在VFA的情况下，生物体积极繁殖。
Both streptomycin-treated and untreated Swiss white mice were irregularly colonized when challenged orogastrically with between 1 and 1011 viable organisms of either of two strains of Campylobacter jejuni. The organisms were occasionally recovered from portions of the intestinal tracts of these animals in numbers ranging from 101 to 103/g when the challenge doses were 1010 or more. When germfree mice were challenged with 108 organisms of either strain, the entire intestinal tracts of all the animals were colonized with C. jejuni in numbers ranging from 104 to 109/g. The ceca were most heavily colonized. Both strains of C. jejuni multiplied anaerobically in brucella broth, except when the broth contained 60.80 .mu.eq of total volatile fatty acids (VFA) per ml at pH 6.75, simulating conditions in the ceca of untreated mice, or when it contained 21.63 .mu.eq/ml at pH 7.04, simulating conditions in the ceca of streptomycin-treated mice. Active multiplication occurred, however, in brucella broth without VFA at pH 7.02 that was incubated microaerobically, simulating conditions in the ceca of germfree mice. The results suggest that VFA operating under anaerobic conditions present in the intestinal tract of both streptomycin-treated and untreatd conventional mice interfere with the multiplication of C. jejuni. The organisms actively multiply, on the other hand, in the absence of VFA at the higher oxidation-reduction potential of the intestinal tract of germfree mice.