Hypoxia-inducible factor 1 alpha (HIF-1α) gene expression in human ovarian carcinoma
Hypoxia-inducible factor 1 alpha (HIF-1α) gene expression in human ovarian carcinoma
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DOI：
10.1016/s0304-3835(01)00762-5
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发表时间：
2002-02-25
期刊：
影响因子：
9.7
通讯作者：
Takebayashi, Y
中科院分区：
文献类型：
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作者：
Nakayama, K;Kanzaki, A;Takebayashi, Y
缺氧诱导因子 1α (HIF-1α) 调节参与缺氧反应并促进新血管生成的基因，是血管内皮细胞生长因子 (VEGF) 的转录因子。本研究的目的是检查人类卵巢癌患者中 HIF-1α 和 VEGF 基因表达及其与血管生成、临床病理变量和生存的关系。我们使用逆转录酶聚合酶链反应 (RT-PCR) 回顾性分析了 60 例卵巢癌中的 HIF-1α 和 VEGF 基因表达水平。使用冰冻切片中的抗 CD 31 抗体通过免疫染色内皮细胞来评估瘤内微血管密度 (IMD)。通过学生 t 检验和卡方检验评估这些基因的表达水平、IMD 和临床病理变量之间的关系。通过Kaplan-Meier曲线进行生存分析。除肿瘤分级外，HIF-1α或VEGF基因表达水平与年龄、临床分期和组织学亚型无关。所有癌症中 HIF-1α 或 VEGF 基因表达水平与 IMD 之间均不存在相关性（R 分别 = 0.118 和 0.224）。此外，还观察到 HIF-1α 和 VEGF 基因表达水平之间存在弱关联（R = 0.300，P = 0,020）。观察到 VEGF 基因表达与 IMD 之间的关联（R = 0.501，P = 0.016）。然而，没有观察到 IMD 和 HIF-1α 基因表达之间的关联。此外，HIF-1α和VEGF基因表达水平对卵巢癌患者的生存没有影响。这些结果表明，HIF-1α基因上调的VEGF可能参与了某些类型卵巢癌的血管生成，但两种基因的表达水平对卵巢癌患者的生存没有影响。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
Hypoxia-inducible factor1alpha (HIF-1alpha)that regulates genes involved in response to hypoxia and promotes neo-angiogenesis, is a transcriptional factor for vascular endothelial cell growth factor (VEGF). The aim of this study was to examine the expression of HIF-1alpha and VEGF gene expressions and their relation to angiogenesis, clinicopathologic variables and survival in the patient with human ovarian carcinoma. We retrospectively analyzed HIF-1alpha and VEGF gene expression levels using reverse transcriptase polymerase chain reaction (RT-PCR) in 60 ovarian carcinomas. Intratumoral microvessel density (IMD) was assessed by immunostaining endothelial cells, using anti-CD 31 antibody in frozen sections. The relationships between the expression level of these genes, IMD and clnicopathologic variables were evaluated by Student's t-test and chi-square tests. Survival analysis was performed by Kaplan-Meier curves. HIF-1alpha or VEGF gene expression level was independent of age, clinical stage and histological subtype besides grade of tumor. There was no relationship between HIF-1alpha or VEGF gene expression level and IMD in all carcinomas (R = 0.118 and 0.224, respectively). In addition, a weak association between HIF-1alpha and VEGF gene expression level was observed (R = 0.300, P = 0,020). The association between VEGF gene expression and IMD was observed (R = 0.501, P = 0.016). However, no association between IMD and HIF-1alpha gene expression was observed. Further, both HIF-1alpha and VEGF gene expression levels had no effect on survival in the patient with ovarian carcinoma. These results suggest that VEGF upregulated by HIF-1alpha gene may be involved in angiogenesis of some type of ovarian carcinoma, but the expression levels of both genes have no effect on survival in the patients with ovarian carcinoma. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.