Sub-150 nm mesoporous silica nanoparticles with tunable pore sizes and well-ordered mesostructure for protein encapsulation
Sub-150 nm mesoporous silica nanoparticles with tunable pore sizes and well-ordered mesostructure for protein encapsulation
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亚 150 nm 介孔二氧化硅纳米粒子，具有可调的孔径和有序的介观结构，用于蛋白质封装
DOI：
10.1016/j.jcis.2013.06.028
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发表时间：
2013-10-01
影响因子：
9.9
通讯作者：
Shi, Jianlin
中科院分区：
文献类型：
--
作者：
Gu, Jinlou;Huang, Kai;Shi, Jianlin
尽管介孔二氧化硅纳米颗粒作为生物大分子载体的潜力很大，但同时具有大孔径、小颗粒直径和有序介孔结构的报道很少。本文以阳离子表面活性剂(CS)为模板剂，N，N-二甲基十六胺(DMHA)为中性胺，F127三嵌段共聚物(EO106PO70EO106)为粒子生长抑制/分散剂，设计了一种简便的单分散MSN的合成方法。得到的胶体纳米粒子具有高度有序的介观结构，孔径可调至4.6 nm(BJH)，单分散粒子尺寸小于150 nm。一种细胞色素c(CytC)的模型蛋白被应用于合成的MSN中，其负载量与其孔径有关。癌细胞对大孔MSN的有效摄取预示着它们作为膜不通透蛋白的细胞内输送载体的潜力。(C)2013 Elsevier Inc.保留所有权利。
Despite their great potentials as biomacromolecues delivery vehicles, there are few, if any, reports on mesoporous silica nanoparticles (MSNs) simultaneously integrated with the merits of large pore size, small particle diameters and well-ordered mesostructure. Here, we designed a facile strategy for the synthesis of monodispersed MSNs using cationic surfactants (CSs) as templating agents, neutral amine of N,N-dimethylhexadecylamine (DMHA) as a pore size mediator and tri-block copolymer of F127 (EO106PO70EO106) as a particle growth inhibitor/dispersant. The obtained colloidal nanoparticles exhibited a highly ordered mesostructure and tunable pore diameter up to 4.6 nm (BJH) and monodispersed particle sizes less than 150 nm. A model protein of cytochrome c (CytC) was exemplified to be accommodated in the resultant MSNs and its loading amount was correlated with their pore size. The efficient cancer cellular uptake of the large-pore MSNs prefigured their potentials as intracellular delivery vehicles for membrane-impermeable proteins. (C) 2013 Elsevier Inc. All rights reserved.