Graphene Oxide Quantum Dots as Novel Nanozymes for Alcohol Intoxication
Graphene Oxide Quantum Dots as Novel Nanozymes for Alcohol Intoxication
复制标题
氧化石墨烯量子点作为新型纳米酶治疗酒精中毒
DOI：
10.1021/acsami.7b00306
复制
发表时间：
2017-04-12
影响因子：
9.5
通讯作者：
Hu, Xiangang
中科院分区：
文献类型：
--
作者：
Sun, Anqi;Mu, Li;Hu, Xiangang
过量饮酒是一个世界性的问题，会导致酒精性肝病(ALD)，如脂肪变性、酒精性肝炎和肝硬化。ALD的治疗已经得到了广泛的研究，但仍然具有挑战性。在这项工作中，发现了氧化石墨烯量子点(GOQds)作为一种新型的纳米酶对酒精过度消费的保护作用，并通过省略分析阐明了这些作用的具体机制。GOQds可以显著缓解乙醇引起的细胞活力下降，并可作为纳米酶加速乙醇代谢，避免有毒中间体在细胞内积累。GOQDS可减轻线粒体损伤和自由基过量产生。细胞保护作用的机制也与代谢和蛋白质信号的改变有关，特别是与脂质代谢有关的信号。GOQD诱导的适度增加的自噬解释了累积的脂类的去除和随后过量的GOQD的消除。这些发现表明，GOQD具有对抗乙醇不良反应的能力，并为GOQD的直接应用提供了新的见解。除了传统的抗氧化外，这项工作还建立了代谢组学和蛋白质组学技术作为发现纳米锌的多重功能的有效工具。
Alcohol overconsumption as a worldwide issue results in alcoholic liver disease (ALD), such as steatosis, alcoholic hepatitis, and cirrhosis. The treatment of ALD has been widely investigated but remains challenging. In this work, the protective effects of graphene oxide quantum dots (GOQDs) as novel nanozymes against alcohol overconsumption are discovered, and the specific mechanisms underlying these effects are elucidated via omits analysis. GOQDs dramatically alleviate the reduction of cell viability induced by ethanol and can act as nanozymes to accelerate ethanol metabolism and avoid the accumulation of toxic intermediates in cells. Mitochondrial damage and the excessive generation of free radicals were mitigated by GOQDs. The mechanisms underlying the cellular protective effects were also related to alterations in metabolic and protein signals, especially those involved in lipid metabolism. The moderately increased autophagy induced by GOQDs explained the removal of accumulated lipids and the subsequent elimination of excessive GOQDs. These findings suggest that GOQDs have an antagonistic capacity against the adverse effects caused by ethanol and provide new insights into the direct applications of GOQDs. In addition to traditional antioxidation, this work also establishes metabolomics and proteomics techniques as effective tools to discover the multiple functions of nanozynies.