Construction of the common cortical space by spontaneous activity and its application in the mouse cortex.
Construction of the common cortical space by spontaneous activity and its application in the mouse cortex.
复制标题
自发活动构建共同皮质空间及其在小鼠皮质中的应用。
DOI：
10.1016/j.bbrc.2019.04.048
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Kunugi H.
中科院分区：
文献类型：
--
作者：
O'Hashi K;Sohya K;Matsuno H;Tsuchimine S;Kunugi H.
动物脑的宽视野光学成像是测量脑动力学（包括空间结构）的有用技术。然而，定量动物间的比较是困难的，由于缺乏共同的皮质空间，可以正常化个别成像的大脑，如在人类功能性MRI研究。在这里，通过使用宽场功能性钙离子成像的麻醉转基因小鼠表达G-CaMP 7的星形胶质细胞和兴奋性中子，我们试图建立小鼠的共同皮层空间，这可能是有用的功能脑地图的标准。我们最初重建了嵌入自发活动中的皮层区域作为个体小鼠的功能连接图，然后通过几何变换在大小，形状和位置上匹配它们。最后，我们将所有记录的信号分配到变换的空间中，以在共同的皮层空间中进行空间归一化。使用这种方法，我们设法提取了小鼠中常见的活动模式。这些结果表明，所提出的方法可用于促进动物间的脑动力学比较，并有可能确定跨动物的共同大脑活动。
Wide-field optical imaging of the animal brain is a useful technique for measuring brain dynamics, including spatial structure. However, quantitative inter-animal comparison is difficult due to lack of the common cortical space that can normalize individually imaged brains as done in human functional MRI studies. Here, by using wide-field functional Ca2+imaging on anesthetized transgenic mice expressing G-CaMP7 in astrocytes and excitatory neutrons, we attempted to establish the common cortical space in mice, which can be useful as a standard of functional brain map. We initially reconstructed cortical areas embedded within spontaneous activity as the functional connectivity maps for the individual mice, then matched them in size, shape, and location across mice by geometric transformation. Finally, we assigned all the recorded signals into the transformed space, to make spatially normalized signals in the common cortical space. Using this method, we managed to extract activity patterns commonly observed across mice. These results suggest that the presented method is available to facilitate inter-animal comparison of brain dynamics, and has the potential to identify common brain activity across animals.