Structural elucidation of a pectin from roots of Polygala tenuifolia and its neuritogenesis inducing activity in PC12 cells
Structural elucidation of a pectin from roots of Polygala tenuifolia and its neuritogenesis inducing activity in PC12 cells
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远志根部果胶的结构解析及其在 PC12 细胞中诱导神经突发生的活性
DOI：
10.1016/j.carbpol.2020.116048
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发表时间：
2020
影响因子：
11.2
通讯作者：
Ding Kan
中科院分区：
文献类型：
--
作者：
Zeng Hui;Huang Lulin;Tao Hong;Zhang Yiwen;Ding Kan
果胶是一类杂多聚糖，因其多种生物活性而受到高度重视。本研究从荆芥根中纯化得到分子量为79.1 kDa的果胶RP01-1。其结构表征为1,2,4 -链α-Rhapand 1,4 -链α-GalpA交替构成骨架，末端(T) -, 1,4 -和1,3,6 -链β-Galp的分支，T-, 1,5 -和1,3,5 -链α- arafp取代1,2,4 -链α-Rhap的C-4。荧光光谱分析表明，RP01-1诱导PC12细胞和原代皮层神经元的神经突生长呈剂量依赖性，无明显细胞毒性。为了揭示这一现象的机制，我们发现RP01-1可能通过增加BDNF的表达，促进AKT、ERK、CREB的磷酸化来诱导PC12细胞的神经新生。结果提示AKT、ERK、CREB相关信号通路可能参与RP01-1诱导神经生成的活性。
Pectin is a family of heteropolysaccharides and highly valued for its various bioactivities. Here, a pectin, RP01-1, was purified from roots ofPolygala tenuifoliawith the molecular weight of 79.1 kDa. Its structure was characterized as alternate 1, 2, 4-linked α-Rhapand 1, 4-linked α-GalpA constituted the backbone, with branches of terminal (T) -, 1, 4- and 1, 3, 6-linked β-Galp, T-, 1, 5- and 1, 3, 5-linked α-Arafsubstituted at C-4 of 1, 2, 4-linked α-Rhap. The fluorescence spectroscopic analysis indicated that RP01-1 induced neurite outgrowth in PC12 cells and primary cortex neurons in dose dependent manner with no significant cytotoxicity. To disclose the mechanism of this phenomenon, we found that RP01-1 might induce neuritogenesis in PC12 cells via increasing BDNF expression, and promoting the phosphorylation of AKT, ERK, CREB. The results suggest that AKT, ERK, CREB related signaling pathways might implicate in neuritogenesis-inducing activity of RP01-1.