An rmlA gene encoding D-glucose-1-phosphate thymidylyltransferase is essential for mycobacterial growth
An rmlA gene encoding D-glucose-1-phosphate thymidylyltransferase is essential for mycobacterial growth
复制标题
DOI：
10.1111/j.1574-6968.2007.00890.x
复制
发表时间：
2007-10-01
影响因子：
2.1
通讯作者：
Ma, Yufang
中科院分区：
文献类型：
--
作者：
Qu, Hong;Xin, Yi;Ma, Yufang
鼠李糖-GlcNAc二糖是一种关键的连接物，它通过磷酸二酯键将阿拉伯半乳糖与肽聚糖连接起来。DTDP-鼠李糖的生物合成由四种酶催化，第一反应由编码D-葡萄糖-1-磷酸胸苷转移酶(RMLA)的RMLA基因催化。我们通过同源重组的方法获得了一株缺失RMLA基因的耻垢分枝杆菌MC(2)155突变体。我们测试了对RMLA基因的要求以及缺乏RMLA对细菌细胞形态的影响。结果表明，RMLA基因对分枝杆菌的生长是必需的，缺乏RMLA活性对细菌细胞形态有深刻的负面影响。因此，RMLA是开发新的抗结核药物的潜在靶点。
The rhamnose-GlcNAc disaccharide is a critical linker which connects arabinogalactan to peptidoglycan via a phosphodiester linkage. The biosynthesis of dTDP-rhamnose is catalysed by four enzymes, and the first reaction is catalysed by an rmlA gene encoding D-glucose-1-phosphate thymidylyltransferase (RmlA). We generated a Mycobacterium smegmatis mc(2)155 mutant lacking the rmlA gene via a homologous recombination method. We tested the requirement for the rmlA gene and the effect of a lack of RmlA on bacterial cell morphology. The results demonstrate that the rmlA gene is essential for mycobacterial growth and that lack of RmlA activity has profound negative effects on bacterial cell morphology. RmlA is thus a potential target for the development of new antituberculosis drugs.