Label-Free Photoelectrochemical "Off-On" Platform Coupled with G-Wire-Enhanced Strategy for Highly Sensitive MicroRNA Sensing in Cancer Cells
Label-Free Photoelectrochemical "Off-On" Platform Coupled with G-Wire-Enhanced Strategy for Highly Sensitive MicroRNA Sensing in Cancer Cells
复制标题
无标记光电化学“Off-On”平台与 G-Wire 增强策略相结合，可在癌细胞中实现高度灵敏的 MicroRNA 传感
DOI：
10.1021/acs.analchem.7b03150
复制
发表时间：
2017
影响因子：
7.4
通讯作者：
Li Nian Bing
中科院分区：
文献类型：
--
作者：
Ye Cui;Wang Min Qiang;Luo Hong Qun;Li Nian Bing
microRNA（miRNAs）的定量，特别是在低丰度时，对于疾病诊断、预后和治疗至关重要。在本文中，我们开发了一种独特的无标记的“关-开”配置用于光电化学（PEC）传感平台制造，与DNA四向结（4J）结构以及用于信号放大的G线超结构相结合。此外，包覆Au纳米颗粒的磷酸铜纳米片（CuPi NS）（Au-CuPi NS）用作高效光电阴极衬底。为了提高灵敏度，并避免假阳性信号，猝灭剂，金纳米颗粒（GNP），被用来关闭PEC信号，因为值得称赞的表面等离子体共振（SPR）吸收。随后，巧妙的DNA 4J架构应用于输出成比例的c-myc区域用于靶点定量。在G-丝超结构形成的辅助下，将增强子5，10，15，20-四（4-磺基苯基）-21H，23H-卟啉（TSPP）偶联到底物上，开启PEC信号，从而实现准确、灵敏、低检测限的miRNA检测。此外，我们在前列腺癌细胞系22 Rv 1中进行了miRNA检测，并获得了理想的定量能力。值得注意的是，所制备的PEC传感平台不仅实现了高效的miRNA定量，而且为传感平台的制造开辟了一个奇妙的前景。
MicroRNA (miRNAs) quantification, especially at low abundance, is vital for disease diagnosis, prognosis, and therapy. Herein we develop a distinctive label-free “off–on” configuration for photoelectrochemical (PEC) sensing platform fabrication, coupled with DNA four-way junction (4J) architecture as well as G-wire superstructure for signal amplification. In addition, ultrathin copper phosphate nanosheets (CuPi NSs) coating Au nanoparticles (Au-CuPi NSs) serve as a highly efficient photocathode substrate. To improve the sensitivity, and avoid the false positive signals, the quencher, gold nanoparticles (GNPs), is utilized to switch off the PEC signal because of the commendable surface plasmon resonance (SPR) absorption. Subsequently, ingenious DNA 4J architecture is applied to export proportional c-myc regions for target quantification. Assisted with the G-wire superstructure formation, the enhancer 5,10,15,20-tetra(4-sulfophenyl)-21H,23H-porphyrin (TSPP) is coupled on the substrate to switch on the PEC signal, thus realizing the miRNA assay with persuasive accuracy, high sensitivity, and low detection limit. In addition, we execute the miRNA detection in prostate carcinoma cell line 22Rv1, and acquire desirable quantitative capability. Remarkably, the prepared PEC sensing platform not only realizes the highly efficient miRNAs quantification, but also uncovers a marvelous horizon for sensing platform fabrication.